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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pelotomaculum thermopropionicum S-adenosylmethionine synthase (metK) CSB-YP398175PYG
CSB-EP398175PYG
CSB-BP398175PYG
CSB-MP398175PYG
CSB-EP398175PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP398176PYG
CSB-EP398176PYG
CSB-BP398176PYG
CSB-MP398176PYG
CSB-EP398176PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP398177PYG
CSB-EP398177PYG
CSB-BP398177PYG
CSB-MP398177PYG
CSB-EP398177PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Protease HtpX homolog (htpX), partial CSB-YP398178PYG1
CSB-EP398178PYG1
CSB-BP398178PYG1
CSB-MP398178PYG1
CSB-EP398178PYG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Transcriptional repressor NrdR (nrdR) CSB-YP398179PYG
CSB-EP398179PYG
CSB-BP398179PYG
CSB-MP398179PYG
CSB-EP398179PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Lipoprotein signal peptidase (lspA), partial CSB-YP398180PYG1
CSB-EP398180PYG1
CSB-BP398180PYG1
CSB-MP398180PYG1
CSB-EP398180PYG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Aspartate carbamoyltransferase (pyrB) CSB-YP398181PYG
CSB-EP398181PYG
CSB-BP398181PYG
CSB-MP398181PYG
CSB-EP398181PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP398182PYG
CSB-EP398182PYG
CSB-BP398182PYG
CSB-MP398182PYG
CSB-EP398182PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum GTPase Der (der) CSB-YP398183PYG
CSB-EP398183PYG
CSB-BP398183PYG
CSB-MP398183PYG
CSB-EP398183PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 7-cyano-7-deazaguanine synthase (queC) CSB-YP398184PYG
CSB-EP398184PYG
CSB-BP398184PYG
CSB-MP398184PYG
CSB-EP398184PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum LexA repressor (lexA) CSB-YP398185PYG
CSB-EP398185PYG
CSB-BP398185PYG
CSB-MP398185PYG
CSB-EP398185PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Ribonuclease Y (rny) CSB-YP398186PYG
CSB-EP398186PYG
CSB-BP398186PYG
CSB-MP398186PYG
CSB-EP398186PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Ribosome-binding factor A (rbfA) CSB-YP398187PYG
CSB-EP398187PYG
CSB-BP398187PYG
CSB-MP398187PYG
CSB-EP398187PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Arginine repressor (argR) CSB-YP398188PYG
CSB-EP398188PYG
CSB-BP398188PYG
CSB-MP398188PYG
CSB-EP398188PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Elongation factor P (efp) CSB-YP398189PYG
CSB-EP398189PYG
CSB-BP398189PYG
CSB-MP398189PYG
CSB-EP398189PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum D-tyrosyl-tRNA (Tyr) deacylase CSB-YP398190PYG
CSB-EP398190PYG
CSB-BP398190PYG
CSB-MP398190PYG
CSB-EP398190PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Redox-sensing transcriptional repressor rex (rex) CSB-YP398191PYG
CSB-EP398191PYG
CSB-BP398191PYG
CSB-MP398191PYG
CSB-EP398191PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP398192PYG
CSB-EP398192PYG
CSB-BP398192PYG
CSB-MP398192PYG
CSB-EP398192PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Maf-like protein PTH_0815 (PTH_0815) CSB-YP398193PYG
CSB-EP398193PYG
CSB-BP398193PYG
CSB-MP398193PYG
CSB-EP398193PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 2-isopropylmalate synthase (leuA), partial CSB-YP398194PYG
CSB-EP398194PYG
CSB-BP398194PYG
CSB-MP398194PYG
CSB-EP398194PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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