重組蛋白

重組蛋白是解析生物相互作用的關(guān)鍵工具,廣泛應(yīng)用于各類(lèi)研究實(shí)驗(yàn)中。無(wú)論是商業(yè)化產(chǎn)品,還是為特定研究定制的蛋白,它們都在多種科學(xué)應(yīng)用中發(fā)揮著不可替代的作用。
若您需要優(yōu)質(zhì)重組蛋白——已驗(yàn)證的生物活性、低內(nèi)毒素水平、高純度——來(lái)為您的研究提供可靠支持,這里正是您的理想之選。
歷經(jīng)18年積累,華美生物已構(gòu)建覆蓋原核與真核的五大蛋白質(zhì)表達(dá)系統(tǒng)。我們擁有包括桿狀病毒外膜蛋白(OMP)表達(dá)技術(shù)、膜蛋白表達(dá)技術(shù)等在內(nèi)的多項(xiàng)蛋白表達(dá)與純化專(zhuān)有技術(shù),致力于為您提供高質(zhì)量的重組蛋白解決方案。
產(chǎn)品特點(diǎn)
- 覆蓋廣泛:擁有超過(guò)16,000種重組蛋白,涵蓋人類(lèi)、小鼠、大鼠、兔、豚鼠、雞、猴、豬等多種物種的基因與靶點(diǎn),支持跨物種比較研究。
- 表達(dá)系統(tǒng)全面:提供五大表達(dá)系統(tǒng),包括大腸桿菌、酵母、桿狀病毒-昆蟲(chóng)細(xì)胞、哺乳動(dòng)物細(xì)胞以及特有的無(wú)細(xì)胞表達(dá)系統(tǒng)。該系統(tǒng)省去質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、破碎離心等步驟,顯著提升蛋白制備效率。
- 多標(biāo)簽選擇:支持His、GST、Flag、MBP等多種標(biāo)簽,便于后續(xù)純化、檢測(cè)與功能研究。
- 多領(lǐng)域應(yīng)用:適用于抗原制備、細(xì)胞與分析、蛋白互作、藥物研發(fā)、酶活性檢測(cè)、ELISA標(biāo)準(zhǔn)品、體內(nèi)外研究、質(zhì)譜與結(jié)構(gòu)分析等廣泛場(chǎng)景。
- 無(wú)動(dòng)物源成分:我們已建立全程無(wú)動(dòng)物副產(chǎn)品接觸的重組蛋白生產(chǎn)線,確保產(chǎn)品的高純凈性與一致性,適用于對(duì)來(lái)源敏感的各類(lèi)實(shí)驗(yàn)。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的重組蛋白,請(qǐng)查看華美生物蛋白定制服務(wù)。
通過(guò)選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
| 產(chǎn)品名稱 | 貨號(hào) | 規(guī)格 |
|---|---|---|
| Recombinant Pelotomaculum thermopropionicum Acylphosphatase (acyP) | CSB-YP399176PYG CSB-EP399176PYG CSB-BP399176PYG CSB-MP399176PYG CSB-EP399176PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pelotomaculum thermopropionicum D-alanine--D-alanine ligase (ddl) | CSB-YP399177PYG CSB-EP399177PYG CSB-BP399177PYG CSB-MP399177PYG CSB-EP399177PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pelotomaculum thermopropionicum Protein hfq (hfq) | CSB-YP399178PYG CSB-EP399178PYG CSB-BP399178PYG CSB-MP399178PYG CSB-EP399178PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pelotomaculum thermopropionicum Proline--tRNA ligase (proS), partial | CSB-YP399179PYG CSB-EP399179PYG CSB-BP399179PYG CSB-MP399179PYG CSB-EP399179PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pelotomaculum thermopropionicum ATP-dependent protease ATPase subunit HslU (hslU) | CSB-YP399180PYG CSB-EP399180PYG CSB-BP399180PYG CSB-MP399180PYG CSB-EP399180PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pelotomaculum thermopropionicum Putative Holliday junction resolvase (PTH_1065) | CSB-YP399181PYG CSB-EP399181PYG CSB-BP399181PYG CSB-MP399181PYG CSB-EP399181PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pelotomaculum thermopropionicum S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) | CSB-YP399182PYG CSB-EP399182PYG CSB-BP399182PYG CSB-MP399182PYG CSB-EP399182PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pelotomaculum thermopropionicum Glutamate-1-semialdehyde 2,1-aminomutase (hemL) | CSB-YP399183PYG CSB-EP399183PYG CSB-BP399183PYG CSB-MP399183PYG CSB-EP399183PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pelotomaculum thermopropionicum Probable rRNA maturation factor (PTH_0892) | CSB-YP399184PYG CSB-EP399184PYG CSB-BP399184PYG CSB-MP399184PYG CSB-EP399184PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pelotomaculum thermopropionicum Elongation factor 4 (lepA), partial | CSB-YP399185PYG CSB-EP399185PYG CSB-BP399185PYG CSB-MP399185PYG CSB-EP399185PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pelotomaculum thermopropionicum Alanine racemase (alr) | CSB-YP399186PYG CSB-EP399186PYG CSB-BP399186PYG CSB-MP399186PYG CSB-EP399186PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pelotomaculum thermopropionicum Phosphoglucosamine mutase (glmM) | CSB-YP399187PYG CSB-EP399187PYG CSB-BP399187PYG CSB-MP399187PYG CSB-EP399187PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pelotomaculum thermopropionicum 30S ribosomal protein S13 (rpsM) | CSB-YP399188PYG CSB-EP399188PYG CSB-BP399188PYG CSB-MP399188PYG CSB-EP399188PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L24 (rplX) | CSB-YP399189PYG CSB-EP399189PYG CSB-BP399189PYG CSB-MP399189PYG CSB-EP399189PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L10 (rplJ) | CSB-YP399190PYG CSB-EP399190PYG CSB-BP399190PYG CSB-MP399190PYG CSB-EP399190PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L23 (rplW) | CSB-YP399191PYG CSB-EP399191PYG CSB-BP399191PYG CSB-MP399191PYG CSB-EP399191PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pelotomaculum thermopropionicum Pantothenate synthetase (panC) | CSB-YP399192PYG CSB-EP399192PYG CSB-BP399192PYG CSB-MP399192PYG CSB-EP399192PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Pelotomaculum thermopropionicum 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) | CSB-YP399193PYG CSB-EP399193PYG CSB-BP399193PYG CSB-MP399193PYG CSB-EP399193PYG-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Human Fat storage-inducing transmembrane protein 1 (FITM1), partial | CSB-YP399194HU1 CSB-EP399194HU1 CSB-BP399194HU1 CSB-MP399194HU1 CSB-EP399194HU1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bovine Spermatogenesis-defective protein 39 homolog (SPE39) | CSB-YP399195BO CSB-EP399195BO CSB-BP399195BO CSB-MP399195BO CSB-EP399195BO-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達(dá)載體?
表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。
根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022






