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Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium thermocellum Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP385186DUT
CSB-EP385186DUT
CSB-BP385186DUT
CSB-MP385186DUT
CSB-EP385186DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Ribosomal protein L11 methyltransferase (prmA) CSB-YP385187DUT
CSB-EP385187DUT
CSB-BP385187DUT
CSB-MP385187DUT
CSB-EP385187DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Chaperone protein DnaK (dnaK), partial CSB-YP385188DUT
CSB-EP385188DUT
CSB-BP385188DUT
CSB-MP385188DUT
CSB-EP385188DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Histidine--tRNA ligase (hisS) CSB-YP385189DUT
CSB-EP385189DUT
CSB-BP385189DUT
CSB-MP385189DUT
CSB-EP385189DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 30S ribosomal protein S21 (rpsU) CSB-YP385190DUT
CSB-EP385190DUT
CSB-BP385190DUT
CSB-MP385190DUT
CSB-EP385190DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Xyloglucanase Xgh74A (xghA), partial CSB-YP385191DUT
CSB-EP385191DUT
CSB-BP385191DUT
CSB-MP385191DUT
CSB-EP385191DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 30S ribosomal protein S9 (rpsI) CSB-YP385192DUT
CSB-EP385192DUT
CSB-BP385192DUT
CSB-MP385192DUT
CSB-EP385192DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP385193DUT
CSB-EP385193DUT
CSB-BP385193DUT
CSB-MP385193DUT
CSB-EP385193DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum ATP-dependent helicase/deoxyribonuclease subunit B (addB), partial CSB-YP385194DUT
CSB-EP385194DUT
CSB-BP385194DUT
CSB-MP385194DUT
CSB-EP385194DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum V-type ATP synthase alpha chain (atpA), partial CSB-YP385195DUT
CSB-EP385195DUT
CSB-BP385195DUT
CSB-MP385195DUT
CSB-EP385195DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum V-type ATP synthase subunit D (atpD) CSB-YP385196DUT
CSB-EP385196DUT
CSB-BP385196DUT
CSB-MP385196DUT
CSB-EP385196DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Ribosomal RNA small subunit methyltransferase G (rsmG) CSB-YP385197DUT
CSB-EP385197DUT
CSB-BP385197DUT
CSB-MP385197DUT
CSB-EP385197DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum tRNA modification GTPase MnmE (mnmE) CSB-YP385198DUT
CSB-EP385198DUT
CSB-BP385198DUT
CSB-MP385198DUT
CSB-EP385198DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum DNA replication and repair protein recF (recF) CSB-YP385199DUT
CSB-EP385199DUT
CSB-BP385199DUT
CSB-MP385199DUT
CSB-EP385199DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Uracil phosphoribosyltransferase (upp) CSB-YP385200DUT
CSB-EP385200DUT
CSB-BP385200DUT
CSB-MP385200DUT
CSB-EP385200DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum ATP synthase gamma chain (atpG) CSB-YP385201DUT
CSB-EP385201DUT
CSB-BP385201DUT
CSB-MP385201DUT
CSB-EP385201DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP385202DUT
CSB-EP385202DUT
CSB-BP385202DUT
CSB-MP385202DUT
CSB-EP385202DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Bifunctional protein GlmU (glmU) CSB-YP385203DUT
CSB-EP385203DUT
CSB-BP385203DUT
CSB-MP385203DUT
CSB-EP385203DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP385204DUT
CSB-EP385204DUT
CSB-BP385204DUT
CSB-MP385204DUT
CSB-EP385204DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum ATP-dependent Clp protease proteolytic subunit (clpP) CSB-YP385205DUT
CSB-EP385205DUT
CSB-BP385205DUT
CSB-MP385205DUT
CSB-EP385205DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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