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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Clostridium thermocellum Cellulose 1,4-beta-cellobiosidase (celK), partial CSB-YP385166DUT
CSB-EP385166DUT
CSB-BP385166DUT
CSB-MP385166DUT
CSB-EP385166DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP385167DUT
CSB-EP385167DUT
CSB-BP385167DUT
CSB-MP385167DUT
CSB-EP385167DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Ribonuclease H (rnhA) CSB-YP385168DUT
CSB-EP385168DUT
CSB-BP385168DUT
CSB-MP385168DUT
CSB-EP385168DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP385169DUT
CSB-EP385169DUT
CSB-BP385169DUT
CSB-MP385169DUT
CSB-EP385169DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 50S ribosomal protein L19 (rplS) CSB-YP385170DUT
CSB-EP385170DUT
CSB-BP385170DUT
CSB-MP385170DUT
CSB-EP385170DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 30S ribosomal protein S16 (rpsP) CSB-YP385171DUT
CSB-EP385171DUT
CSB-BP385171DUT
CSB-MP385171DUT
CSB-EP385171DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum DNA mismatch repair protein MutS (mutS), partial CSB-YP385172DUT
CSB-EP385172DUT
CSB-BP385172DUT
CSB-MP385172DUT
CSB-EP385172DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 3-dehydroquinate synthase (aroB) CSB-YP385173DUT
CSB-EP385173DUT
CSB-BP385173DUT
CSB-MP385173DUT
CSB-EP385173DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Endoglucanase D (celD), partial CSB-YP385174DUT
CSB-EP385174DUT
CSB-BP385174DUT
CSB-MP385174DUT
CSB-EP385174DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP385175DUT
CSB-EP385175DUT
CSB-BP385175DUT
CSB-MP385175DUT
CSB-EP385175DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Aspartate 1-decarboxylase (panD) CSB-YP385176DUT
CSB-EP385176DUT
CSB-BP385176DUT
CSB-MP385176DUT
CSB-EP385176DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Aspartate carbamoyltransferase (pyrB) CSB-YP385177DUT
CSB-EP385177DUT
CSB-BP385177DUT
CSB-MP385177DUT
CSB-EP385177DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase CSB-YP385178DUT
CSB-EP385178DUT
CSB-BP385178DUT
CSB-MP385178DUT
CSB-EP385178DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Ribosome-binding factor A (rbfA) CSB-YP385179DUT
CSB-EP385179DUT
CSB-BP385179DUT
CSB-MP385179DUT
CSB-EP385179DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum MutS2 protein (mutS2), partial CSB-YP385180DUT
CSB-EP385180DUT
CSB-BP385180DUT
CSB-MP385180DUT
CSB-EP385180DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum GTPase Der (der) CSB-YP385181DUT
CSB-EP385181DUT
CSB-BP385181DUT
CSB-MP385181DUT
CSB-EP385181DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP385182DUT
CSB-EP385182DUT
CSB-BP385182DUT
CSB-MP385182DUT
CSB-EP385182DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Bifunctional protein FolD (folD) CSB-YP385183DUT
CSB-EP385183DUT
CSB-BP385183DUT
CSB-MP385183DUT
CSB-EP385183DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum 5-methylthioadenosine/S-adenosylhomocysteine deaminase (mtaD) CSB-YP385184DUT
CSB-EP385184DUT
CSB-BP385184DUT
CSB-MP385184DUT
CSB-EP385184DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP385185DUT
CSB-EP385185DUT
CSB-BP385185DUT
CSB-MP385185DUT
CSB-EP385185DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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