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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Paraclostridium sordellii 7alpha-hydroxysteroid dehydrogenase CSB-EP341870CMG 1mg/100ug/20ug
Recombinant Cat Beta-lactoglobulin-2 (LGB2) CSB-EP322005CA 1mg/100ug/20ug
Recombinant Human CDGSH iron-sulfur domain-containing protein 3, mitochondrial (CISD3) CSB-EP005444HU 1mg/100ug/20ug
Recombinant Influenza A virus RNA-directed RNA polymerase catalytic subunit (PB1), partial CSB-EP361052IFZ 1mg/100ug/20ug
Recombinant Influenza A virus Polymerase basic protein 2 (PB2), partial CSB-EP361051IFZ 1mg/100ug/20ug
Recombinant Mouse Glutathione peroxidase 3 (Gpx3) (U73S) CSB-EP009868MO(M) 1mg/100ug/20ug
Recombinant Dog NK3 homeobox 2 (NKX3-2), partial CSB-EP7059DO 1mg/100ug/20ug
Recombinant Escherichia coli O6:H1 Isocitrate lyase (aceA) CSB-EP359295EGX 1mg/100ug/20ug
Recombinant Human Thrombospondin type-1 domain-containing protein 7A (THSD7A), partial CSB-EP891984HU 1mg/100ug/20ug
Recombinant Human Transforming growth factor beta-1 proprotein (TGFB1)(C33S), partial CSB-MP023446HU2(M) 1mg/100ug/20ug
Recombinant Human Epidermal growth factor receptor (EGFR), partial, Biotinylated CSB-MP007479HUj10-B 1mg/100ug/20ug
Recombinant Human Tumor necrosis factor receptor superfamily member 13C (TNFRSF13C)(L27A,V29T,H31S), partial CSB-MP853495HU2(M1)-C 1mg/100ug/20ug
Recombinant Human Tumor necrosis factor receptor superfamily member 13C (TNFRSF13C)(V29L,H31S), partial CSB-MP853495HU2(M2)-C 1mg/100ug/20ug
Recombinant Rat Advanced glycosylation end product-specific receptor (Ager), partial CSB-MP001441RA-C 1mg/100ug/20ug
Recombinant Human Triggering receptor expressed on myeloid cells 1 (TREM-1), partial CSB-MP024404HU 1mg/100ug/20ug
Recombinant Human Alpha-2-antiplasmin (SERPINF2) CSB-MP021085HU 1mg/100ug/20ug
Recombinant Sus scrofa CCN family member 4 (CCN4) CSB-MP7652PI 1mg/100ug/20ug
Recombinant Human Zinc finger Y-chromosomal protein (ZFY), partial CSB-MP026468HU 1mg/100ug/20ug
Recombinant Human Acylglycerol kinase, mitochondrial (AGK) CSB-MP706634HU 1mg/100ug/20ug
Recombinant Sus scrofa CCN family member 4 (CCN family member 4) CSB-MP7652PId9 1mg/100ug/20ug

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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