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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus subtilis Uncharacterized protein yunG (yunG) CSB-YP523372BRJ
CSB-EP523372BRJ
CSB-BP523372BRJ
CSB-MP523372BRJ
CSB-EP523372BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uric acid degradation bifunctional protein PucL (pucL) CSB-YP523373BRJ
CSB-BP523373BRJ
CSB-MP523373BRJ
CSB-EP523373BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable xanthine dehydrogenase subunit C (pucC) CSB-YP523374BRJ
CSB-EP523374BRJ
CSB-BP523374BRJ
CSB-MP523374BRJ
CSB-EP523374BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Allantoate amidohydrolase (pucF) CSB-YP523375BRJ
CSB-EP523375BRJ
CSB-BP523375BRJ
CSB-MP523375BRJ
CSB-EP523375BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yurQ (yurQ) CSB-YP523376BRJ
CSB-EP523376BRJ
CSB-BP523376BRJ
CSB-MP523376BRJ
CSB-EP523376BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable cysteine desulfurase (csd) CSB-YP523377BRJ
CSB-EP523377BRJ
CSB-BP523377BRJ
CSB-MP523377BRJ
CSB-EP523377BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Methionine import ATP-binding protein MetN (metN) CSB-YP523378BRJ
CSB-EP523378BRJ
CSB-BP523378BRJ
CSB-MP523378BRJ
CSB-EP523378BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Short-chain dehydrogenase/reductase homolog yusS (yusS) CSB-YP523379BRJ
CSB-EP523379BRJ
CSB-BP523379BRJ
CSB-MP523379BRJ
CSB-EP523379BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yusW (yusW) CSB-YP523380BRJ
CSB-EP523380BRJ
CSB-BP523380BRJ
CSB-MP523380BRJ
CSB-EP523380BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Protein liaF (liaF), partial CSB-YP523381BRJ1
CSB-EP523381BRJ1
CSB-BP523381BRJ1
CSB-MP523381BRJ1
CSB-EP523381BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative arginine/ornithine antiporter (yvsH), partial CSB-YP523382BRJ
CSB-EP523382BRJ
CSB-BP523382BRJ
CSB-MP523382BRJ
CSB-EP523382BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative molybdenum transport system permease protein yvgM (yvgM), partial CSB-YP523383BRJ1
CSB-EP523383BRJ1
CSB-BP523383BRJ1
CSB-MP523383BRJ1
CSB-EP523383BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sulfite reductase [NADPH] flavoprotein alpha-component (cysJ), partial CSB-YP523384BRJ
CSB-EP523384BRJ
CSB-BP523384BRJ
CSB-MP523384BRJ
CSB-EP523384BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Cadmium, zinc and cobalt-transporting ATPase (cadA), partial CSB-YP523385BRJ
CSB-EP523385BRJ
CSB-BP523385BRJ
CSB-MP523385BRJ
CSB-EP523385BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein yvaC (yvaC), partial CSB-YP523386BRJ
CSB-EP523386BRJ
CSB-BP523386BRJ
CSB-MP523386BRJ
CSB-EP523386BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized oxidoreductase yvaG (yvaG) CSB-YP523387BRJ
CSB-EP523387BRJ
CSB-BP523387BRJ
CSB-MP523387BRJ
CSB-EP523387BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis AB hydrolase superfamily protein yvaM (yvaM) CSB-YP523388BRJ
CSB-EP523388BRJ
CSB-BP523388BRJ
CSB-MP523388BRJ
CSB-EP523388BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative sensory transducer protein yvaQ (yvaQ), partial CSB-YP523389BRJ1
CSB-EP523389BRJ1
CSB-BP523389BRJ1
CSB-MP523389BRJ1
CSB-EP523389BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yvbH (yvbH) CSB-YP523390BRJ
CSB-EP523390BRJ
CSB-BP523390BRJ
CSB-MP523390BRJ
CSB-EP523390BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yvbT (yvbT) CSB-YP523391BRJ
CSB-EP523391BRJ
CSB-BP523391BRJ
CSB-MP523391BRJ
CSB-EP523391BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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