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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus subtilis Putative dipeptidase ytjP (ytjP) CSB-YP523573BRJ
CSB-EP523573BRJ
CSB-BP523573BRJ
CSB-MP523573BRJ
CSB-EP523573BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sensory transduction protein BceR (bceR) CSB-YP523574BRJ
CSB-EP523574BRJ
CSB-BP523574BRJ
CSB-MP523574BRJ
CSB-EP523574BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yjaV (yjaV) CSB-YP523575BRJ
CSB-EP523575BRJ
CSB-BP523575BRJ
CSB-MP523575BRJ
CSB-EP523575BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Probable zinc transport system zinc-binding lipoprotein AdcA (adcA) CSB-YP523576BRJ
CSB-EP523576BRJ
CSB-BP523576BRJ
CSB-MP523576BRJ
CSB-EP523576BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rathayibacter rathayi Sensor protein KdpD (kdpD), partial CSB-YP523577RJU
CSB-EP523577RJU
CSB-BP523577RJU
CSB-MP523577RJU
CSB-EP523577RJU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Guanine/hypoxanthine permease PbuO (pbuO), partial CSB-YP523578BRJ
CSB-EP523578BRJ
CSB-BP523578BRJ
CSB-MP523578BRJ
CSB-EP523578BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized hydrolase YtnL (ytnL) CSB-YP523579BRJ
CSB-EP523579BRJ
CSB-BP523579BRJ
CSB-MP523579BRJ
CSB-EP523579BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yvnB (yvnB), partial CSB-YP523580BRJ
CSB-EP523580BRJ
CSB-BP523580BRJ
CSB-MP523580BRJ
CSB-EP523580BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Putative acetyltransferase yvoF (yvoF) CSB-YP523581BRJ
CSB-EP523581BRJ
CSB-BP523581BRJ
CSB-MP523581BRJ
CSB-EP523581BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis ABC transporter permease ytrF (ytrF), partial CSB-YP523582BRJ1
CSB-EP523582BRJ1
CSB-BP523582BRJ1
CSB-MP523582BRJ1
CSB-EP523582BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Gamma-D-glutamyl-L-lysine endopeptidase (ykfC) CSB-YP523583BRJ
CSB-EP523583BRJ
CSB-BP523583BRJ
CSB-MP523583BRJ
CSB-EP523583BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized EAL-domain containing protein ykuI (ykuI) CSB-YP523584BRJ
CSB-EP523584BRJ
CSB-BP523584BRJ
CSB-MP523584BRJ
CSB-EP523584BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0435 protein yfkK (yfkK) CSB-YP523585BRJ
CSB-EP523585BRJ
CSB-BP523585BRJ
CSB-MP523585BRJ
CSB-EP523585BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Type-2 restriction enzyme BsuMI component ydiR (ydiR) CSB-YP523586BRJ
CSB-EP523586BRJ
CSB-BP523586BRJ
CSB-MP523586BRJ
CSB-EP523586BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Acylphosphatase (acyP) CSB-YP523587BRJ
CSB-EP523587BRJ
CSB-BP523587BRJ
CSB-MP523587BRJ
CSB-EP523587BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized metallophosphoesterase ykoQ (ykoQ) CSB-YP523588BRJ
CSB-EP523588BRJ
CSB-BP523588BRJ
CSB-MP523588BRJ
CSB-EP523588BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized zinc-type alcohol dehydrogenase-like protein YjmD (yjmD) CSB-YP523589BRJ
CSB-EP523589BRJ
CSB-BP523589BRJ
CSB-MP523589BRJ
CSB-EP523589BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Prolactin-4A1 (Prl4a1) CSB-YP523590MO
CSB-EP523590MO
CSB-BP523590MO
CSB-MP523590MO
CSB-EP523590MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Eosinophil cationic-type ribonuclease 3 (Ear3) CSB-YP523591MO
CSB-EP523591MO
CSB-BP523591MO
CSB-MP523591MO
CSB-EP523591MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 6 (Hsd3b6), partial CSB-YP523592MO1
CSB-EP523592MO1
CSB-BP523592MO1
CSB-MP523592MO1
CSB-EP523592MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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