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Human Platelet-Derived Growth Factor AB,PDGF-AB ELISA kit

  • 中文名稱:
    人血血小板衍生生長(zhǎng)因子AB(PDGF-AB)酶聯(lián)免疫試劑盒
  • 貨號(hào):
    CSB-E04701h
  • 樣本類型:
    serum, plasma, tissue homogenates
  • 檢測(cè)范圍:
    31.25 pg/mL-2000 pg/mL
  • 靈敏度:
    7.8 pg/mL
  • 規(guī)格:
    96T/48T
  • 價(jià)格:
    ¥3200/¥2500
  • 促銷:
  • 其他:

產(chǎn)品詳情

  • 產(chǎn)品描述:
    人血血小板衍生生長(zhǎng)因子AB(PDGF-AB)酶聯(lián)免疫試劑盒(CSB-E04701h)為雙抗夾心法ELISA試劑盒,定量檢測(cè)血清、血漿、組織勻漿樣本中的PDGF-AB含量。PDGF-AB是一種由血小板分泌的生長(zhǎng)因子,由A和B鏈組成,參與調(diào)控纖維母細(xì)胞和平滑肌細(xì)胞等中胚層來(lái)源細(xì)胞的增殖和遷移。其研究機(jī)制涉及PDGF與PDGFRα和PDGFRβ受體的結(jié)合,激活下游信號(hào)通路,促進(jìn)細(xì)胞增殖和血管生成。試劑盒檢測(cè)范圍為31.25 pg/mL-2000 pg/mL,靈敏度為7.8 pg/mL。適用于體外實(shí)驗(yàn)中對(duì)PDGF-AB的定量分析,例如探究心血管疾病模型中的信號(hào)通路機(jī)制、評(píng)估藥物干預(yù)對(duì)細(xì)胞因子表達(dá)的影響,或通過(guò)組織樣本分析疾病相關(guān)分子標(biāo)志物變化,為細(xì)胞生物學(xué)、病理學(xué)及藥理學(xué)研究提供可靠數(shù)據(jù)支持。本品僅用于科研,不用于臨床診斷,產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書(shū)。
  • 縮寫:
    PDGF-AB
  • 種屬:
    Homo sapiens (Human)
  • 反應(yīng)時(shí)間:
    1-5h
  • 上樣量:
    50-100ul
  • 檢測(cè)波長(zhǎng):
    450 nm
  • 研究領(lǐng)域:
    Cancer
  • 測(cè)定原理:
    quantitative
  • 測(cè)定方法:
    Sandwich
  • 精密度:

     Intra-assay Precision (Precision within an assay): CV%<8%

    Three samples of known concentration were tested twenty times on one plate to assess. 
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess. 
     
  • 線性度:

     To assess the linearity of the assay, samples were spiked with high concentrations of human PDGF-AB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

  • 回收率:

     The recovery of human PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

  • 標(biāo)準(zhǔn)曲線:

     These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 

  • 數(shù)據(jù)處理:
  • 貨期:
    3-5 working days

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

  • 最新研究進(jìn)展:
    PDGF-AB是血小板源性生長(zhǎng)因子(platelet-derived growth factor,PDGF)家族的一個(gè)亞型,由PDGF-A和PDGF-B兩個(gè)亞基組成。最近的研究表明,PDGF-AB參與了多種生物學(xué)過(guò)程,如細(xì)胞增殖、分化、遷移和細(xì)胞外基質(zhì)合成等。此外,PDGF-AB在多種疾病中也發(fā)揮著重要作用,如腫瘤、動(dòng)脈粥樣硬化和組織損傷等。因此,PDGF-AB成為了治療靶點(diǎn)的研究熱點(diǎn)。一些研究已經(jīng)探索了PDGF-AB的抑制劑和抗體,以期望發(fā)現(xiàn)更有效的治療方案。


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