Western Blot
Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, Rat kidney tissue
All lanes: PTPN12 antibody at 7.4µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 89, 73, 75 kDa
Observed band size: 89 kDa

IHC image of CSB-PA019026LA01HU diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.