Western Blot
Positive WB detected in: HL60 whole cell lysate, SH-SY5Y whole cell lysate, Mouse liver tissue
All lanes: PTGS1 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 69, 65, 62, 57, 72, 73 kDa
Observed band size: 72 kDa

Overlay histogram showing Hela cells stained with CSB-RA918236A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1μg/1*10⁶ cells) for 1 h at 4℃.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4℃. Control antibody (green line) was Rabbit IgG (1μg/1*10⁶ cells) used under the same conditions. Acquisition of >10,000 events was performed.