Western Blot
Positive WB detected in: U-251MG whole cell lysate(20μg),HT-29 whole cell lysate(20μg), NIH/3T3 whole cell lysate(20μg)
All lanes: MTAP antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDa
Exposure time：1min20s

Immunofluorescence staining of Hela cell with CSB-RA299565A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Overlay Peak curve showing Hela cells stained with CSB-RA299565A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate, Mouse liver tissue, Mouse kidney tissue
All lanes: MTAP antibody at 2.7μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 32, 39, 37, 31, 34, 33, 27 kDa
Observed band size: 32, 39 kDa

Immunofluorescent analysis of U251 cells using CSB-PA622639LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)