Western Blot
Positive WB detected in: HepG2 whole cell lysate(30μg), U251 whole cell lysate(30μg), A549 whole cell lysate(30μg), Hela whole cell lysate(30μg), MCF7 whole cell lysate(30μg),K562 whole cell lysate(30μg), Mouse Heart tissue lysate(30μg),Mouse Liver tissue lysate(30μg),Rat Heart tissue lysate(30μg)
All lanes: DGAT2 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 40,44 kDa
Observed band size: 44 kDa
Exposure time: 120s

IHC image of CSB-PA850407LA01HU diluted at 1:50 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody