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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila ananassae Serine protease HTRA2, mitochondrial (HtrA2), partial CSB-YP453406DKY1
CSB-EP453406DKY1
CSB-BP453406DKY1
CSB-MP453406DKY1
CSB-EP453406DKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Forkhead box protein O (foxo), partial CSB-YP453407DKY
CSB-EP453407DKY
CSB-BP453407DKY
CSB-MP453407DKY
CSB-EP453407DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Structure-specific endonuclease subunit SLX1 homolog (slx1) CSB-YP453408DKY
CSB-EP453408DKY
CSB-BP453408DKY
CSB-MP453408DKY
CSB-EP453408DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Histone-arginine methyltransferase CARMER (Art4), partial CSB-YP453409DKY
CSB-EP453409DKY
CSB-BP453409DKY
CSB-MP453409DKY
CSB-EP453409DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Mesencephalic astrocyte-derived neurotrophic factor homolog (Manf) CSB-YP453410DKY
CSB-EP453410DKY
CSB-BP453410DKY
CSB-MP453410DKY
CSB-EP453410DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Lipoyl synthase, mitochondrial (Las), partial CSB-YP453411DKY
CSB-EP453411DKY
CSB-BP453411DKY
CSB-MP453411DKY
CSB-EP453411DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae ATPase ASNA1 homolog (GF11712) CSB-YP453412DKY
CSB-EP453412DKY
CSB-BP453412DKY
CSB-MP453412DKY
CSB-EP453412DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Protein KIAA0664 homolog (GF12179), partial CSB-YP453413DKY
CSB-EP453413DKY
CSB-BP453413DKY
CSB-MP453413DKY
CSB-EP453413DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Adenosine monophosphate-protein transferase FICD homolog (GF14521), partial CSB-YP453414DKY1
CSB-EP453414DKY1
CSB-BP453414DKY1
CSB-MP453414DKY1
CSB-EP453414DKY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Glutamyl-tRNA (Gln) amidotransferase subunit C, mitochondrial CSB-YP453415DKY
CSB-EP453415DKY
CSB-BP453415DKY
CSB-MP453415DKY
CSB-EP453415DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae NFU1 iron-sulfur cluster scaffold homolog, mitochondrial (GF20932) CSB-YP453416DKY
CSB-EP453416DKY
CSB-BP453416DKY
CSB-MP453416DKY
CSB-EP453416DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Transcription factor kayak (kay), partial CSB-YP453417DKY
CSB-EP453417DKY
CSB-BP453417DKY
CSB-MP453417DKY
CSB-EP453417DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae NAD (P)H-hydrate epimerase CSB-YP453418DKY
CSB-EP453418DKY
CSB-BP453418DKY
CSB-MP453418DKY
CSB-EP453418DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Anamorsin homolog (l (2)35Bg) CSB-YP453419DLK
CSB-EP453419DLK
CSB-BP453419DLK
CSB-MP453419DLK
CSB-EP453419DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Putative N (4)- (beta-N-acetylglucosaminyl)-L-asparaginase GG24090 CSB-YP453420DLK
CSB-EP453420DLK
CSB-BP453420DLK
CSB-MP453420DLK
CSB-EP453420DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Latrophilin Cirl (Cirl), partial CSB-YP453421DLK
CSB-EP453421DLK
CSB-BP453421DLK
CSB-MP453421DLK
CSB-EP453421DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Protein cueball (cue), partial CSB-YP453422DLK
CSB-EP453422DLK
CSB-BP453422DLK
CSB-MP453422DLK
CSB-EP453422DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta SAGA-associated factor 11 homolog (Sgf11) CSB-YP453423DLK
CSB-EP453423DLK
CSB-BP453423DLK
CSB-MP453423DLK
CSB-EP453423DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Protein king tubby (king-tubby) CSB-YP453424DLK
CSB-EP453424DLK
CSB-BP453424DLK
CSB-MP453424DLK
CSB-EP453424DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Protein CLP1 homolog (cbc) CSB-YP453425DLK
CSB-EP453425DLK
CSB-BP453425DLK
CSB-MP453425DLK
CSB-EP453425DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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