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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Drosophila ananassae Enhancer of yellow 2 transcription factor (e (y)2) CSB-YP451465DKY
CSB-EP451465DKY
CSB-BP451465DKY
CSB-MP451465DKY
CSB-EP451465DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Sodium-dependent nutrient amino acid transporter 1 (NAAT1), partial CSB-YP451466DKY
CSB-EP451466DKY
CSB-BP451466DKY
CSB-MP451466DKY
CSB-EP451466DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Protein phosphatase PTC7 homolog fig (fig) CSB-YP451467DKY
CSB-EP451467DKY
CSB-BP451467DKY
CSB-MP451467DKY
CSB-EP451467DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Vitelline membrane protein Vm32E (Vm32E) CSB-YP451468DLK
CSB-EP451468DLK
CSB-BP451468DLK
CSB-MP451468DLK
CSB-EP451468DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Protein crossbronx (cbx) CSB-YP451469DLK
CSB-EP451469DLK
CSB-BP451469DLK
CSB-MP451469DLK
CSB-EP451469DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta SOSS complex subunit C homolog (GG16026) CSB-YP451470DLK
CSB-EP451470DLK
CSB-BP451470DLK
CSB-MP451470DLK
CSB-EP451470DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Cytosolic Fe-S cluster assembly factor NUBP2 homolog (GG16127) CSB-YP451471DLK
CSB-EP451471DLK
CSB-BP451471DLK
CSB-MP451471DLK
CSB-EP451471DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Protein Turandot E (Victoria) CSB-YP451472DLK
CSB-EP451472DLK
CSB-BP451472DLK
CSB-MP451472DLK
CSB-EP451472DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Adenylate kinase 2, mitochondrial (Adk2) CSB-YP451473DLK
CSB-EP451473DLK
CSB-BP451473DLK
CSB-MP451473DLK
CSB-EP451473DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Tyrosine-protein kinase-like otk (otk), partial CSB-YP451474DLK
CSB-EP451474DLK
CSB-BP451474DLK
CSB-MP451474DLK
CSB-EP451474DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta DM7 family protein GG17593 (GG17593), partial CSB-YP451475DLK
CSB-EP451475DLK
CSB-BP451475DLK
CSB-MP451475DLK
CSB-EP451475DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Werner Syndrome-like exonuclease (WRNexo) CSB-YP451476DLK
CSB-EP451476DLK
CSB-BP451476DLK
CSB-MP451476DLK
CSB-EP451476DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Male-specific protein scotti (soti) CSB-YP451477DLK
CSB-EP451477DLK
CSB-BP451477DLK
CSB-MP451477DLK
CSB-EP451477DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Protein cereblon homolog (GG17313), partial CSB-YP451478DLK
CSB-EP451478DLK
CSB-BP451478DLK
CSB-MP451478DLK
CSB-EP451478DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Telomere-binding protein cav (cav) CSB-YP451479DLK
CSB-EP451479DLK
CSB-BP451479DLK
CSB-MP451479DLK
CSB-EP451479DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Succinyl-diaminopimelate desuccinylase (dapE) CSB-YP451480DQY
CSB-EP451480DQY
CSB-BP451480DQY
CSB-MP451480DQY
CSB-EP451480DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus 30S ribosomal protein S2 (rpsB) CSB-YP451481DQY
CSB-EP451481DQY
CSB-BP451481DQY
CSB-MP451481DQY
CSB-EP451481DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Lipid-A-disaccharide synthase (lpxB) CSB-YP451482DQY
CSB-EP451482DQY
CSB-BP451482DQY
CSB-MP451482DQY
CSB-EP451482DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus 7-cyano-7-deazaguanine synthase (queC) CSB-YP451483DQY
CSB-EP451483DQY
CSB-BP451483DQY
CSB-MP451483DQY
CSB-EP451483DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (lpxC) CSB-YP451484DQY
CSB-EP451484DQY
CSB-BP451484DQY
CSB-MP451484DQY
CSB-EP451484DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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