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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus weihenstephanensis MutS2 protein (mutS2), partial CSB-YP433292BON
CSB-EP433292BON
CSB-BP433292BON
CSB-MP433292BON
CSB-EP433292BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP433293BON
CSB-EP433293BON
CSB-BP433293BON
CSB-MP433293BON
CSB-EP433293BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis UPF0173 metal-dependent hydrolase BcerKBAB4_4442 (BcerKBAB4_4442) CSB-YP433294BON
CSB-EP433294BON
CSB-BP433294BON
CSB-MP433294BON
CSB-EP433294BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis N- (5'-phosphoribosyl)anthranilate isomerase CSB-YP433295BON
CSB-EP433295BON
CSB-BP433295BON
CSB-MP433295BON
CSB-EP433295BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Glutamate 5-kinase (proB) CSB-YP433296BON
CSB-EP433296BON
CSB-BP433296BON
CSB-MP433296BON
CSB-EP433296BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Septation ring formation regulator EzrA (ezrA), partial CSB-YP433297BON
CSB-EP433297BON
CSB-BP433297BON
CSB-MP433297BON
CSB-EP433297BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis D-alanine--poly (phosphoribitol) ligase subunit 2 CSB-YP433298BON
CSB-EP433298BON
CSB-BP433298BON
CSB-MP433298BON
CSB-EP433298BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Imidazoleglycerol-phosphate dehydratase (hisB) CSB-YP433299BON
CSB-EP433299BON
CSB-BP433299BON
CSB-MP433299BON
CSB-EP433299BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP433300BON
CSB-EP433300BON
CSB-BP433300BON
CSB-MP433300BON
CSB-EP433300BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Methylglyoxal synthase (mgsA) CSB-YP433301BON
CSB-EP433301BON
CSB-BP433301BON
CSB-MP433301BON
CSB-EP433301BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Cell cycle protein GpsB (gpsB) CSB-YP433302BON
CSB-EP433302BON
CSB-BP433302BON
CSB-MP433302BON
CSB-EP433302BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Probable cytosol aminopeptidase (pepA) CSB-YP433303BON
CSB-EP433303BON
CSB-BP433303BON
CSB-MP433303BON
CSB-EP433303BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Putative ATP:guanido phosphotransferase BcerKBAB4_0075 (BcerKBAB4_0075) CSB-YP433304BON
CSB-EP433304BON
CSB-BP433304BON
CSB-MP433304BON
CSB-EP433304BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Cysteine--tRNA ligase (cysS) CSB-YP433305BON
CSB-EP433305BON
CSB-BP433305BON
CSB-MP433305BON
CSB-EP433305BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP433306BON
CSB-EP433306BON
CSB-BP433306BON
CSB-MP433306BON
CSB-EP433306BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L22 (rplV) CSB-YP433307BON
CSB-EP433307BON
CSB-BP433307BON
CSB-MP433307BON
CSB-EP433307BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 30S ribosomal protein S17 (rpsQ) CSB-YP433308BON
CSB-EP433308BON
CSB-BP433308BON
CSB-MP433308BON
CSB-EP433308BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 30S ribosomal protein S14 type Z (rpsZ) CSB-YP433309BON
CSB-EP433309BON
CSB-BP433309BON
CSB-MP433309BON
CSB-EP433309BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis 50S ribosomal protein L17 (rplQ) CSB-YP433310BON
CSB-EP433310BON
CSB-BP433310BON
CSB-MP433310BON
CSB-EP433310BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus weihenstephanensis Pyrophosphatase ppaX (ppaX) CSB-YP433311BON
CSB-EP433311BON
CSB-BP433311BON
CSB-MP433311BON
CSB-EP433311BON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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