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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanoregula boonei D-tyrosyl-tRNA (Tyr) deacylase CSB-YP418323MNV
CSB-EP418323MNV
CSB-BP418323MNV
CSB-MP418323MNV
CSB-EP418323MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei NH (3)-dependent NAD (+) synthetase CSB-YP418324MNV
CSB-EP418324MNV
CSB-BP418324MNV
CSB-MP418324MNV
CSB-EP418324MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoregula boonei V-type ATP synthase alpha chain (atpA), partial CSB-YP418325MNV
CSB-EP418325MNV
CSB-BP418325MNV
CSB-MP418325MNV
CSB-EP418325MNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Dihydroorotate dehydrogenase (quinone) CSB-YP418326XAV
CSB-EP418326XAV
CSB-BP418326XAV
CSB-MP418326XAV
CSB-EP418326XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Ribosome maturation factor RimP (rimP) CSB-YP418327XAV
CSB-EP418327XAV
CSB-BP418327XAV
CSB-MP418327XAV
CSB-EP418327XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP418328XAV
CSB-EP418328XAV
CSB-BP418328XAV
CSB-MP418328XAV
CSB-EP418328XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 4-hydroxy-2-oxovalerate aldolase (Xaut_0938) CSB-YP418329XAV
CSB-EP418329XAV
CSB-BP418329XAV
CSB-MP418329XAV
CSB-EP418329XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus UDP-N-acetylmuramate--L-alanine ligase (murC) CSB-YP418330XAV
CSB-EP418330XAV
CSB-BP418330XAV
CSB-MP418330XAV
CSB-EP418330XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 30S ribosomal protein S16 (rpsP) CSB-YP418331XAV
CSB-EP418331XAV
CSB-BP418331XAV
CSB-MP418331XAV
CSB-EP418331XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 30S ribosomal protein S7 (rpsG) CSB-YP418332XAV
CSB-EP418332XAV
CSB-BP418332XAV
CSB-MP418332XAV
CSB-EP418332XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 50S ribosomal protein L16 (rplP) CSB-YP418333XAV
CSB-EP418333XAV
CSB-BP418333XAV
CSB-MP418333XAV
CSB-EP418333XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus UPF0391 membrane protein Xaut_1725 (Xaut_1725), partial CSB-YP418334XAV
CSB-EP418334XAV
CSB-BP418334XAV
CSB-MP418334XAV
CSB-EP418334XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Protein MraZ (mraZ) CSB-YP418335XAV
CSB-EP418335XAV
CSB-BP418335XAV
CSB-MP418335XAV
CSB-EP418335XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Probable glycine dehydrogenase [decarboxylating] subunit 1 (gcvPA) CSB-YP418336XAV
CSB-EP418336XAV
CSB-BP418336XAV
CSB-MP418336XAV
CSB-EP418336XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus D-amino acid dehydrogenase small subunit (dadA) CSB-YP418337XAV
CSB-EP418337XAV
CSB-BP418337XAV
CSB-MP418337XAV
CSB-EP418337XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Elongation factor P (efp) CSB-YP418338XAV
CSB-EP418338XAV
CSB-BP418338XAV
CSB-MP418338XAV
CSB-EP418338XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 50S ribosomal protein L25 (rplY) CSB-YP418339XAV
CSB-EP418339XAV
CSB-BP418339XAV
CSB-MP418339XAV
CSB-EP418339XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Probable transcriptional regulatory protein Xaut_3062 (Xaut_3062) CSB-YP418340XAV
CSB-EP418340XAV
CSB-BP418340XAV
CSB-MP418340XAV
CSB-EP418340XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus 30S ribosomal protein S6 (rpsF) CSB-YP418341XAV
CSB-EP418341XAV
CSB-BP418341XAV
CSB-MP418341XAV
CSB-EP418341XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthobacter autotrophicus Aspartate--tRNA ligase (aspS), partial CSB-YP418342XAV
CSB-EP418342XAV
CSB-BP418342XAV
CSB-MP418342XAV
CSB-EP418342XAV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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