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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Actinobacillus succinogenes Leucine--tRNA ligase (leuS), partial CSB-YP408800AUG
CSB-EP408800AUG
CSB-BP408800AUG
CSB-MP408800AUG
CSB-EP408800AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Bifunctional protein FolD (folD) CSB-YP408801AUG
CSB-EP408801AUG
CSB-BP408801AUG
CSB-MP408801AUG
CSB-EP408801AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes UPF0352 protein Asuc_0778 (Asuc_0778) CSB-YP408802AUG
CSB-EP408802AUG
CSB-BP408802AUG
CSB-MP408802AUG
CSB-EP408802AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Protein sprT (sprT) CSB-YP408803AUG
CSB-EP408803AUG
CSB-BP408803AUG
CSB-MP408803AUG
CSB-EP408803AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Probable intracellular septation protein A (Asuc_0882), partial CSB-YP408804AUG1
CSB-EP408804AUG1
CSB-BP408804AUG1
CSB-MP408804AUG1
CSB-EP408804AUG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 50S ribosomal protein L21 (rplU) CSB-YP408805AUG
CSB-EP408805AUG
CSB-BP408805AUG
CSB-MP408805AUG
CSB-EP408805AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Serine/threonine transporter sstT (sstT), partial CSB-YP408806AUG
CSB-EP408806AUG
CSB-BP408806AUG
CSB-MP408806AUG
CSB-EP408806AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes UPF0319 protein Asuc_1002 (Asuc_1002) CSB-YP408807AUG
CSB-EP408807AUG
CSB-BP408807AUG
CSB-MP408807AUG
CSB-EP408807AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes CCA-adding enzyme (cca) CSB-YP408808AUG
CSB-EP408808AUG
CSB-BP408808AUG
CSB-MP408808AUG
CSB-EP408808AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP408809AUG
CSB-EP408809AUG
CSB-BP408809AUG
CSB-MP408809AUG
CSB-EP408809AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP408810AUG
CSB-EP408810AUG
CSB-BP408810AUG
CSB-MP408810AUG
CSB-EP408810AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes UPF0263 protein Asuc_1259 (Asuc_1259) CSB-YP408811AUG
CSB-EP408811AUG
CSB-BP408811AUG
CSB-MP408811AUG
CSB-EP408811AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP408812AUG
CSB-EP408812AUG
CSB-BP408812AUG
CSB-MP408812AUG
CSB-EP408812AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Dihydroorotate dehydrogenase (quinone) CSB-YP408813AUG
CSB-EP408813AUG
CSB-BP408813AUG
CSB-MP408813AUG
CSB-EP408813AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Phosphoglucosamine mutase (glmM) CSB-YP408814AUG
CSB-EP408814AUG
CSB-BP408814AUG
CSB-MP408814AUG
CSB-EP408814AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Tryptophan synthase alpha chain (trpA) CSB-YP408815AUG
CSB-EP408815AUG
CSB-BP408815AUG
CSB-MP408815AUG
CSB-EP408815AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Cytidylate kinase (cmk) CSB-YP408816AUG
CSB-EP408816AUG
CSB-BP408816AUG
CSB-MP408816AUG
CSB-EP408816AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Ribosomal RNA large subunit methyltransferase M (rlmM) CSB-YP408817AUG
CSB-EP408817AUG
CSB-BP408817AUG
CSB-MP408817AUG
CSB-EP408817AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Tetraacyldisaccharide 4'-kinase (lpxK) CSB-YP408818AUG
CSB-EP408818AUG
CSB-BP408818AUG
CSB-MP408818AUG
CSB-EP408818AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus succinogenes Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP408819AUG
CSB-EP408819AUG
CSB-BP408819AUG
CSB-MP408819AUG
CSB-EP408819AUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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