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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Brucella ovis Protein hfq (hfq) CSB-YP404478BPI
CSB-EP404478BPI
CSB-BP404478BPI
CSB-MP404478BPI
CSB-EP404478BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP404479BPI
CSB-EP404479BPI
CSB-BP404479BPI
CSB-MP404479BPI
CSB-EP404479BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Alanine--tRNA ligase (alaS), partial CSB-YP404480BPI
CSB-EP404480BPI
CSB-BP404480BPI
CSB-MP404480BPI
CSB-EP404480BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 30S ribosomal protein S5 (rpsE) CSB-YP404481BPI
CSB-EP404481BPI
CSB-BP404481BPI
CSB-MP404481BPI
CSB-EP404481BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 50S ribosomal protein L5 (rplE) CSB-YP404482BPI
CSB-EP404482BPI
CSB-BP404482BPI
CSB-MP404482BPI
CSB-EP404482BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 30S ribosomal protein S3 (rpsC) CSB-YP404483BPI
CSB-EP404483BPI
CSB-BP404483BPI
CSB-MP404483BPI
CSB-EP404483BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 50S ribosomal protein L7/L12 (rplL) CSB-YP404484BPI
CSB-EP404484BPI
CSB-BP404484BPI
CSB-MP404484BPI
CSB-EP404484BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis UPF0303 protein BOV_1367 (BOV_1367) CSB-YP404485BPI
CSB-EP404485BPI
CSB-BP404485BPI
CSB-MP404485BPI
CSB-EP404485BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Trans-aconitate 2-methyltransferase (tam) CSB-YP404486BPI
CSB-EP404486BPI
CSB-BP404486BPI
CSB-MP404486BPI
CSB-EP404486BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis tRNA (Ile)-lysidine synthase CSB-YP404487BPI
CSB-EP404487BPI
CSB-BP404487BPI
CSB-MP404487BPI
CSB-EP404487BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 50S ribosomal protein L31 (rpmE) CSB-YP404488BPI
CSB-EP404488BPI
CSB-BP404488BPI
CSB-MP404488BPI
CSB-EP404488BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis ATP synthase gamma chain (atpG) CSB-YP404489BPI
CSB-EP404489BPI
CSB-BP404489BPI
CSB-MP404489BPI
CSB-EP404489BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 30S ribosomal protein S16 (rpsP) CSB-YP404490BPI
CSB-EP404490BPI
CSB-BP404490BPI
CSB-MP404490BPI
CSB-EP404490BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP404491BPI
CSB-EP404491BPI
CSB-BP404491BPI
CSB-MP404491BPI
CSB-EP404491BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Histidinol-phosphate aminotransferase (hisC) CSB-YP404492BPI
CSB-EP404492BPI
CSB-BP404492BPI
CSB-MP404492BPI
CSB-EP404492BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP404493BPI
CSB-EP404493BPI
CSB-BP404493BPI
CSB-MP404493BPI
CSB-EP404493BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Imidazole glycerol phosphate synthase subunit HisF (hisF) CSB-YP404494BPI
CSB-EP404494BPI
CSB-BP404494BPI
CSB-MP404494BPI
CSB-EP404494BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP404495BPI
CSB-EP404495BPI
CSB-BP404495BPI
CSB-MP404495BPI
CSB-EP404495BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis tRNA pseudouridine synthase B (truB) CSB-YP404496BPI
CSB-EP404496BPI
CSB-BP404496BPI
CSB-MP404496BPI
CSB-EP404496BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella ovis Formamidopyrimidine-DNA glycosylase (mutM) CSB-YP404497BPI
CSB-EP404497BPI
CSB-BP404497BPI
CSB-MP404497BPI
CSB-EP404497BPI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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