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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Pelotomaculum thermopropionicum Phosphoglycerate kinase (pgk) CSB-YP398755PYG
CSB-EP398755PYG
CSB-BP398755PYG
CSB-MP398755PYG
CSB-EP398755PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP398756PYG
CSB-EP398756PYG
CSB-BP398756PYG
CSB-MP398756PYG
CSB-EP398756PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP398757PYG
CSB-EP398757PYG
CSB-BP398757PYG
CSB-MP398757PYG
CSB-EP398757PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Phosphate acyltransferase (plsX) CSB-YP398758PYG
CSB-EP398758PYG
CSB-BP398758PYG
CSB-MP398758PYG
CSB-EP398758PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum tRNA (guanine-N (1)-)-methyltransferase CSB-YP398759PYG
CSB-EP398759PYG
CSB-BP398759PYG
CSB-MP398759PYG
CSB-EP398759PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum UPF0756 membrane protein PTH_1668 (PTH_1668), partial CSB-YP398760PYG1
CSB-EP398760PYG1
CSB-BP398760PYG1
CSB-MP398760PYG1
CSB-EP398760PYG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum DNA polymerase IV (dinB) CSB-YP398761PYG
CSB-EP398761PYG
CSB-BP398761PYG
CSB-MP398761PYG
CSB-EP398761PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum DNA mismatch repair protein MutS (mutS), partial CSB-YP398762PYG
CSB-EP398762PYG
CSB-BP398762PYG
CSB-MP398762PYG
CSB-EP398762PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 30S ribosomal protein S15 (rpsO) CSB-YP398763PYG
CSB-EP398763PYG
CSB-BP398763PYG
CSB-MP398763PYG
CSB-EP398763PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Tyrosine recombinase XerC (xerC) CSB-YP398764PYG
CSB-EP398764PYG
CSB-BP398764PYG
CSB-MP398764PYG
CSB-EP398764PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Bifunctional protein FolD (folD) CSB-YP398765PYG
CSB-EP398765PYG
CSB-BP398765PYG
CSB-MP398765PYG
CSB-EP398765PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Shikimate kinase (aroK) CSB-YP398766PYG
CSB-EP398766PYG
CSB-BP398766PYG
CSB-MP398766PYG
CSB-EP398766PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Alanine--tRNA ligase (alaS), partial CSB-YP398767PYG
CSB-EP398767PYG
CSB-BP398767PYG
CSB-MP398767PYG
CSB-EP398767PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L21 (rplU) CSB-YP398768PYG
CSB-EP398768PYG
CSB-BP398768PYG
CSB-MP398768PYG
CSB-EP398768PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Thiazole synthase (thiG) CSB-YP398769PYG
CSB-EP398769PYG
CSB-BP398769PYG
CSB-MP398769PYG
CSB-EP398769PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 30S ribosomal protein S17 (rpsQ) CSB-YP398770PYG
CSB-EP398770PYG
CSB-BP398770PYG
CSB-MP398770PYG
CSB-EP398770PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 50S ribosomal protein L15 (rplO) CSB-YP398771PYG
CSB-EP398771PYG
CSB-BP398771PYG
CSB-MP398771PYG
CSB-EP398771PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 30S ribosomal protein S12 (rpsL) CSB-YP398772PYG
CSB-EP398772PYG
CSB-BP398772PYG
CSB-MP398772PYG
CSB-EP398772PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum Elongation factor Tu 2 (tuf2) CSB-YP398773PYG
CSB-EP398773PYG
CSB-BP398773PYG
CSB-MP398773PYG
CSB-EP398773PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelotomaculum thermopropionicum 30S ribosomal protein S19 (rpsS) CSB-YP398774PYG
CSB-EP398774PYG
CSB-BP398774PYG
CSB-MP398774PYG
CSB-EP398774PYG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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