重組蛋白
重組蛋白是解析生物相互作用的關(guān)鍵工具，廣泛應用于各類研究實驗中。無論是商業(yè)化產(chǎn)品，還是為特定研究定制的蛋白，它們都在多種科學應用中發(fā)揮著不可替代的作用。
若您需要優(yōu)質(zhì)重組蛋白——已驗證的生物活性、低內(nèi)毒素水平、高純度——來為您的研究提供可靠支持，這里正是您的理想之選。
歷經(jīng)18年積累，華美生物已構(gòu)建覆蓋原核與真核的五大蛋白質(zhì)表達系統(tǒng)。我們擁有包括桿狀病毒外膜蛋白（OMP）表達技術(shù)、膜蛋白表達技術(shù)等在內(nèi)的多項蛋白表達與純化專有技術(shù)，致力于為您提供高質(zhì)量的重組蛋白解決方案。
產(chǎn)品特點
- 覆蓋廣泛：擁有超過16,000種重組蛋白，涵蓋人類、小鼠、大鼠、兔、豚鼠、雞、猴、豬等多種物種的基因與靶點，支持跨物種比較研究。
- 表達系統(tǒng)全面：提供五大表達系統(tǒng)，包括大腸桿菌、酵母、桿狀病毒-昆蟲細胞、哺乳動物細胞以及特有的無細胞表達系統(tǒng)。該系統(tǒng)省去質(zhì)粒轉(zhuǎn)化、細胞培養(yǎng)、破碎離心等步驟，顯著提升蛋白制備效率。
- 多標簽選擇：支持His、GST、Flag、MBP等多種標簽，便于后續(xù)純化、檢測與功能研究。
- 多領域應用：適用于抗原制備、細胞與分析、蛋白互作、藥物研發(fā)、酶活性檢測、ELISA標準品、體內(nèi)外研究、質(zhì)譜與結(jié)構(gòu)分析等廣泛場景。
- 無動物源成分：我們已建立全程無動物副產(chǎn)品接觸的重組蛋白生產(chǎn)線，確保產(chǎn)品的高純凈性與一致性，適用于對來源敏感的各類實驗。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì)，或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的重組蛋白，請查看華美生物蛋白定制服務。
通過選擇目標名稱的首字母瀏覽產(chǎn)品。
| 產(chǎn)品名稱 | 貨號 | 規(guī)格 |
|---|---|---|
| Recombinant Polynucleobacter sp. Probable intracellular septation protein A (Pnuc_0973), partial | CSB-YP391851PYU1 CSB-EP391851PYU1 CSB-BP391851PYU1 CSB-MP391851PYU1 CSB-EP391851PYU1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. Phosphoglucosamine mutase (glmM) | CSB-YP391852PYU CSB-EP391852PYU CSB-BP391852PYU CSB-MP391852PYU CSB-EP391852PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. Carbamoyl-phosphate synthase small chain (carA) | CSB-YP391853PYU CSB-EP391853PYU CSB-BP391853PYU CSB-MP391853PYU CSB-EP391853PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. Polyribonucleotide nucleotidyltransferase (pnp), partial | CSB-YP391854PYU CSB-EP391854PYU CSB-BP391854PYU CSB-MP391854PYU CSB-EP391854PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. Ketol-acid reductoisomerase (ilvC) | CSB-YP391855PYU CSB-EP391855PYU CSB-BP391855PYU CSB-MP391855PYU CSB-EP391855PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. Urease subunit alpha (ureC), partial | CSB-YP391856PYU CSB-EP391856PYU CSB-BP391856PYU CSB-MP391856PYU CSB-EP391856PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. Urease accessory protein UreD (ureD) | CSB-YP391857PYU CSB-EP391857PYU CSB-BP391857PYU CSB-MP391857PYU CSB-EP391857PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. Ribosome maturation factor RimP (rimP) | CSB-YP391858PYU CSB-EP391858PYU CSB-BP391858PYU CSB-MP391858PYU CSB-EP391858PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. Adenylosuccinate synthetase (purA) | CSB-YP391859PYU CSB-EP391859PYU CSB-BP391859PYU CSB-MP391859PYU CSB-EP391859PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. Ribosomal RNA large subunit methyltransferase N (rlmN) | CSB-YP391860PYU CSB-EP391860PYU CSB-BP391860PYU CSB-MP391860PYU CSB-EP391860PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA) | CSB-YP391861PYU CSB-EP391861PYU CSB-BP391861PYU CSB-MP391861PYU CSB-EP391861PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. Succinyl-diaminopimelate desuccinylase (dapE) | CSB-YP391862PYU CSB-EP391862PYU CSB-BP391862PYU CSB-MP391862PYU CSB-EP391862PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. Cytochrome c-type biogenesis protein CcmE (ccmE), partial | CSB-YP391863PYU CSB-EP391863PYU CSB-BP391863PYU CSB-MP391863PYU CSB-EP391863PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. UPF0173 metal-dependent hydrolase Pnuc_1524 (Pnuc_1524) | CSB-YP391864PYU CSB-EP391864PYU CSB-BP391864PYU CSB-MP391864PYU CSB-EP391864PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. 30S ribosomal protein S21 (rpsU) | CSB-YP391865PYU CSB-EP391865PYU CSB-BP391865PYU CSB-MP391865PYU CSB-EP391865PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. 50S ribosomal protein L33 (rpmG) | CSB-YP391866PYU CSB-EP391866PYU CSB-BP391866PYU CSB-MP391866PYU CSB-EP391866PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. S-adenosylmethionine:tRNA ribosyltransferase-isomerase (queA) | CSB-YP391867PYU CSB-EP391867PYU CSB-BP391867PYU CSB-MP391867PYU CSB-EP391867PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. tRNA-specific 2-thiouridylase mnmA (mnmA) | CSB-YP391868PYU CSB-EP391868PYU CSB-BP391868PYU CSB-MP391868PYU CSB-EP391868PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. Probable rRNA maturation factor (Pnuc_1860) | CSB-YP391869PYU CSB-EP391869PYU CSB-BP391869PYU CSB-MP391869PYU CSB-EP391869PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Polynucleobacter sp. Crossover junction endodeoxyribonuclease RuvC (ruvC) | CSB-YP391870PYU CSB-EP391870PYU CSB-BP391870PYU CSB-MP391870PYU CSB-EP391870PYU-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。
第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條：如何選擇合適的表達載體?
表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。
根據(jù)重組蛋白的不同應用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022
