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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Universal stress protein A (uspA) CSB-MP820596SWW 1mg/100ug/20ug
Recombinant Mouse Collagen alpha-1 (XXVI) chain (Col26a1) CSB-BP820987MO 1mg/100ug/20ug
Recombinant Arabidopsis thaliana 2-Cys peroxiredoxin BAS1, chloroplastic (BAS1) CSB-EP822134DOA 1mg/100μg/20μg
Recombinant Human Lymphokine-activated killer T-cell-originated protein kinase (PBK) CSB-EP822241HU 1mg/100ug/20ug
Recombinant Human RING finger and CHY zinc finger domain-containing protein 1 (RCHY1) CSB-EP822281HU 1mg/100ug/20ug
Recombinant Human Uncharacterized protein C20orf197 (C20orf197) CSB-EP822688HU 1mg/100ug/20ug
Recombinant Human Ribonuclease P protein subunit p25-like protein (RPP25L) CSB-EP822707HU 1mg/100μg/20μg
Recombinant Cricetulus griseus Protein disulfide-isomerase (P4HB) CSB-EP823188DXU 1mg/100μg/20μg
Recombinant Human E3 ubiquitin-protein ligase RNF125 (RNF125) CSB-EP836205HU 1mg/100μg/20μg
Recombinant Human BTB/POZ domain-containing protein KCTD15 (KCTD15) CSB-EP836301HU 1mg/100ug/20ug
Recombinant Human Guanosine-3',5'-bis (diphosphate) 3'-pyrophosphohydrolase MESH1 (HDDC3) CSB-EP836666HU 1mg/100ug/20ug
Recombinant Mouse Interleukin-34 (Il34) CSB-EP837125MO 1mg/100μg/20μg
Recombinant Human Splicing factor U2AF 26 kDa subunit (U2AF1L4) CSB-EP837850HU 1mg/100μg/20μg
Recombinant Human Selenoprotein M (SELENOM) CSB-EP837868HU 1mg/100μg/20μg
Recombinant Human Splicing regulatory glutamine/lysine-rich protein 1 (SREK1), partial CSB-EP837871HU 1mg/100μg/20μg
Recombinant Mouse Beta-synuclein (Sncb) CSB-EP838720MO 1mg/100μg/20μg
Recombinant Human Glycine N-acyltransferase-like protein 1 (GLYATL1) CSB-EP839276HU 1mg/100ug/20ug
Recombinant Human Probable methylthioribulose-1-phosphate dehydratase (APIP) CSB-EP839332HU 1mg/100ug/20ug
Recombinant Arabidopsis thaliana Transcriptional activator DEMETER (DME), partial CSB-EP839701DOA 1mg/100μg/20μg
Recombinant Human Casein kinase I isoform alpha-like (CSNK1A1L) CSB-EP839816HU 1mg/100μg/20μg

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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