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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus thuringiensis Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP369935BOM
CSB-EP369935BOM
CSB-BP369935BOM
CSB-MP369935BOM
CSB-EP369935BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Purine nucleoside phosphorylase DeoD-type (deoD) CSB-YP369936BOM
CSB-EP369936BOM
CSB-BP369936BOM
CSB-MP369936BOM
CSB-EP369936BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP369937BOM
CSB-EP369937BOM
CSB-BP369937BOM
CSB-MP369937BOM
CSB-EP369937BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 3-dehydroquinate synthase (aroB) CSB-YP369938BOM
CSB-EP369938BOM
CSB-BP369938BOM
CSB-MP369938BOM
CSB-EP369938BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP369939BOM
CSB-EP369939BOM
CSB-BP369939BOM
CSB-MP369939BOM
CSB-EP369939BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Holliday junction resolvase recU (recU) CSB-YP369940BOM
CSB-EP369940BOM
CSB-BP369940BOM
CSB-MP369940BOM
CSB-EP369940BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Cell cycle protein GpsB (gpsB) CSB-YP369941BOM
CSB-EP369941BOM
CSB-BP369941BOM
CSB-MP369941BOM
CSB-EP369941BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Dihydroxy-acid dehydratase (ilvD), partial CSB-YP369942BOM
CSB-EP369942BOM
CSB-BP369942BOM
CSB-MP369942BOM
CSB-EP369942BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Uncharacterized HTH-type transcriptional regulator BALH_1720 (BALH_1720) CSB-YP369943BOM
CSB-EP369943BOM
CSB-BP369943BOM
CSB-MP369943BOM
CSB-EP369943BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Acetaldehyde dehydrogenase (BALH_1843) CSB-YP369944BOM
CSB-EP369944BOM
CSB-BP369944BOM
CSB-MP369944BOM
CSB-EP369944BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Isoleucine--tRNA ligase (ileS), partial CSB-YP369945BOM
CSB-EP369945BOM
CSB-BP369945BOM
CSB-MP369945BOM
CSB-EP369945BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Methylmalonate semialdehyde dehydrogenase [acylating] 1 (iolA1) CSB-YP369946BOM
CSB-EP369946BOM
CSB-BP369946BOM
CSB-MP369946BOM
CSB-EP369946BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP369947BOM
CSB-EP369947BOM
CSB-BP369947BOM
CSB-MP369947BOM
CSB-EP369947BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione hydrolase, partial CSB-YP369948BOM
CSB-EP369948BOM
CSB-BP369948BOM
CSB-MP369948BOM
CSB-EP369948BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis UPF0457 protein BALH_2270 (BALH_2270) CSB-YP369949BOM
CSB-EP369949BOM
CSB-BP369949BOM
CSB-MP369949BOM
CSB-EP369949BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Undecaprenyl-diphosphatase 4 (uppP4), partial CSB-YP369950BOM
CSB-EP369950BOM
CSB-BP369950BOM
CSB-MP369950BOM
CSB-EP369950BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Chorismate synthase 2 (aroC2) CSB-YP369951BOM
CSB-EP369951BOM
CSB-BP369951BOM
CSB-MP369951BOM
CSB-EP369951BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis UPF0753 protein BALH_2835 (BALH_2835), partial CSB-YP369952BOM
CSB-EP369952BOM
CSB-BP369952BOM
CSB-MP369952BOM
CSB-EP369952BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis Imidazolonepropionase (hutI) CSB-YP369953BOM
CSB-EP369953BOM
CSB-BP369953BOM
CSB-MP369953BOM
CSB-EP369953BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis LexA repressor (lexA) CSB-YP369954BOM
CSB-EP369954BOM
CSB-BP369954BOM
CSB-MP369954BOM
CSB-EP369954BOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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