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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechococcus sp. Ribulose bisphosphate carboxylase large chain (cbbL) CSB-YP309851SVC
CSB-EP309851SVC
CSB-BP309851SVC
CSB-MP309851SVC
CSB-EP309851SVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional regulator lrpC (lrpC) CSB-YP309852BRJ
CSB-EP309852BRJ
CSB-BP309852BRJ
CSB-MP309852BRJ
CSB-EP309852BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydaT (ydaT) CSB-YP309853BRJ
CSB-EP309853BRJ
CSB-BP309853BRJ
CSB-MP309853BRJ
CSB-EP309853BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized ABC transporter ATP-binding protein YdbJ (ydbJ) CSB-YP309854BRJ
CSB-EP309854BRJ
CSB-BP309854BRJ
CSB-MP309854BRJ
CSB-EP309854BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis D-alanine--D-alanine ligase (ddl) CSB-YP309855BRJ
CSB-EP309855BRJ
CSB-BP309855BRJ
CSB-MP309855BRJ
CSB-EP309855BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis mRNA interferase EndoA (ndoA) CSB-YP309856BRJ
CSB-EP309856BRJ
CSB-BP309856BRJ
CSB-MP309856BRJ
CSB-EP309856BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis HTH-type transcriptional regulator immR (immR) CSB-YP309857BRJ
CSB-EP309857BRJ
CSB-BP309857BRJ
CSB-MP309857BRJ
CSB-EP309857BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein yddI (yddI), partial CSB-YP309858BRJ
CSB-EP309858BRJ
CSB-BP309858BRJ
CSB-MP309858BRJ
CSB-EP309858BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized MFS-type transporter yddS (yddS), partial CSB-YP309859BRJ
CSB-EP309859BRJ
CSB-BP309859BRJ
CSB-MP309859BRJ
CSB-EP309859BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ydeB (ydeB) CSB-YP309860BRJ
CSB-EP309860BRJ
CSB-BP309860BRJ
CSB-MP309860BRJ
CSB-EP309860BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized membrane protein ydeH (ydeH), partial CSB-YP309861BRJ1
CSB-EP309861BRJ1
CSB-BP309861BRJ1
CSB-MP309861BRJ1
CSB-EP309861BRJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized N-acetyltransferase YdfB (ydfB) CSB-YP309862BRJ
CSB-EP309862BRJ
CSB-BP309862BRJ
CSB-MP309862BRJ
CSB-EP309862BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Sensor histidine kinase ydfH (ydfH), partial CSB-YP309863BRJ
CSB-EP309863BRJ
CSB-BP309863BRJ
CSB-MP309863BRJ
CSB-EP309863BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Spore coat protein P (cotP) CSB-YP309864BRJ
CSB-EP309864BRJ
CSB-BP309864BRJ
CSB-MP309864BRJ
CSB-EP309864BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized HTH-type transcriptional regulator ydgG (ydgG) CSB-YP309865BRJ
CSB-EP309865BRJ
CSB-BP309865BRJ
CSB-MP309865BRJ
CSB-EP309865BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Arabinose-proton symporter (araE), partial CSB-YP309866BRJ
CSB-EP309866BRJ
CSB-BP309866BRJ
CSB-MP309866BRJ
CSB-EP309866BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Tyrosine-protein kinase YwqD (ywqD) CSB-YP309867BRJ
CSB-EP309867BRJ
CSB-BP309867BRJ
CSB-MP309867BRJ
CSB-EP309867BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis UPF0720 protein ywqJ (ywqJ), partial CSB-YP309868BRJ
CSB-EP309868BRJ
CSB-BP309868BRJ
CSB-MP309868BRJ
CSB-EP309868BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Uncharacterized protein ywsA (ywsA) CSB-YP309869BRJ
CSB-EP309869BRJ
CSB-BP309869BRJ
CSB-MP309869BRJ
CSB-EP309869BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Gamma-DL-glutamyl hydrolase (pgdS) CSB-YP309870BRJ
CSB-EP309870BRJ
CSB-BP309870BRJ
CSB-MP309870BRJ
CSB-EP309870BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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