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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Methionine aminopeptidase 2 (MAP2) CSB-YP013716SVP
CSB-EP013716SVP
CSB-BP013716SVP
CSB-MP013716SVP
CSB-EP013716SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Structure-specific endonuclease subunit SLX4 (SLX4), partial CSB-YP002843SVP
CSB-EP002843SVP
CSB-BP002843SVP
CSB-MP002843SVP
CSB-EP002843SVP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Ubiquitin-related modifier 1 homolog (GF23776) CSB-YP025676DKY
CSB-EP025676DKY
CSB-BP025676DKY
CSB-MP025676DKY
CSB-EP025676DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Eukaryotic translation initiation factor 3 subunit L (GF25000), partial CSB-YP007541DKY
CSB-EP007541DKY
CSB-BP007541DKY
CSB-MP007541DKY
CSB-EP007541DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Eukaryotic translation initiation factor 3 subunit M (Tango7) CSB-YP007542DKY
CSB-EP007542DKY
CSB-BP007542DKY
CSB-MP007542DKY
CSB-EP007542DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Cytoplasmic tRNA 2-thiolation protein 2 (GF15629) CSB-YP003313DKY
CSB-EP003313DKY
CSB-BP003313DKY
CSB-MP003313DKY
CSB-EP003313DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae 40S ribosomal protein SA (sta) CSB-YP020485DKY
CSB-EP020485DKY
CSB-BP020485DKY
CSB-MP020485DKY
CSB-EP020485DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Protein SMG8 (GG10361), partial CSB-YP003375DLK
CSB-EP003375DLK
CSB-BP003375DLK
CSB-MP003375DLK
CSB-EP003375DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta KRR1 small subunit processome component homolog (dbe) CSB-YP012502DLK
CSB-EP012502DLK
CSB-BP012502DLK
CSB-MP012502DLK
CSB-EP012502DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Eukaryotic translation initiation factor 3 subunit L (GG13851), partial CSB-YP007541DLK
CSB-EP007541DLK
CSB-BP007541DLK
CSB-MP007541DLK
CSB-EP007541DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Vacuolar ATPase assembly integral membrane protein VMA21 (GG16118), partial CSB-YP025866DLK1
CSB-EP025866DLK1
CSB-BP025866DLK1
CSB-MP025866DLK1
CSB-EP025866DLK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Eukaryotic translation initiation factor 3 subunit M (Tango7) CSB-YP007542DLK
CSB-EP007542DLK
CSB-BP007542DLK
CSB-MP007542DLK
CSB-EP007542DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Lateral signaling target protein 2 homolog (GG12136), partial CSB-YP026476DLK
CSB-EP026476DLK
CSB-BP026476DLK
CSB-MP026476DLK
CSB-EP026476DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta 40S ribosomal protein S3a (RpS3A) CSB-YP020444DLK
CSB-EP020444DLK
CSB-BP020444DLK
CSB-MP020444DLK
CSB-EP020444DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Malate dehydrogenase (mdh) CSB-YP013623DQY
CSB-EP013623DQY
CSB-BP013623DQY
CSB-MP013623DQY
CSB-EP013623DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Translation initiation factor IF-2 (infB), partial CSB-YP015164DQY
CSB-EP015164DQY
CSB-BP015164DQY
CSB-MP015164DQY
CSB-EP015164DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus ATP synthase subunit beta (atpD) CSB-YP002350DQY
CSB-EP002350DQY
CSB-BP002350DQY
CSB-MP002350DQY
CSB-EP002350DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cellvibrio japonicus Peptide chain release factor 1 (prfA) CSB-YP015197DQY
CSB-EP015197DQY
CSB-BP015197DQY
CSB-MP015197DQY
CSB-EP015197DQY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma arthritidis Elongation factor G (fusA), partial CSB-YP009373MWD
CSB-EP009373MWD
CSB-BP009373MWD
CSB-MP009373MWD
CSB-EP009373MWD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Ribonuclease 3 (rnc) CSB-YP019809RKL
CSB-EP019809RKL
CSB-BP019809RKL
CSB-MP019809RKL
CSB-EP019809RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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