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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Probable calcium-binding protein CML16 (CML16) CSB-YP878495DOA
CSB-EP878495DOA
CSB-BP878495DOA
CSB-MP878495DOA
CSB-EP878495DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/kelch-repeat protein At3g16880 (At3g16880) CSB-YP878496DOA
CSB-EP878496DOA
CSB-BP878496DOA
CSB-MP878496DOA
CSB-EP878496DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Monothiol glutaredoxin-S10 (GRXS10) CSB-YP878497DOA
CSB-EP878497DOA
CSB-BP878497DOA
CSB-MP878497DOA
CSB-EP878497DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Zinc finger CCCH domain-containing protein 38 (At3g18640), partial CSB-YP878498DOA
CSB-EP878498DOA
CSB-BP878498DOA
CSB-MP878498DOA
CSB-EP878498DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/kelch-repeat protein At3g22870 (At3g22870) CSB-YP878499DOA
CSB-EP878499DOA
CSB-BP878499DOA
CSB-MP878499DOA
CSB-EP878499DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g24000, mitochondrial (PCMP-H87), partial CSB-YP878500DOA
CSB-EP878500DOA
CSB-BP878500DOA
CSB-MP878500DOA
CSB-EP878500DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Beta-amylase 1, chloroplastic (BAM1), partial CSB-YP878501DOA 1mg/100ug/20ug
Recombinant Arabidopsis thaliana Zinc finger protein CONSTANS-LIKE 12 (COL12) CSB-YP878502DOA
CSB-EP878502DOA
CSB-BP878502DOA
CSB-MP878502DOA
CSB-EP878502DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana UDP-glycosyltransferase 79B4 (UGT79B4) CSB-YP878503DOA
CSB-EP878503DOA
CSB-BP878503DOA
CSB-MP878503DOA
CSB-EP878503DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Chaperonin 60 subunit beta 2, chloroplastic (CPN60B2), partial CSB-YP878504DOA
CSB-EP878504DOA
CSB-BP878504DOA
CSB-MP878504DOA
CSB-EP878504DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g28660 (PCMP-E80), partial CSB-YP878505DOA
CSB-EP878505DOA
CSB-BP878505DOA
CSB-MP878505DOA
CSB-EP878505DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Magnesium transporter MRS2-3 (MRS2-3), partial CSB-YP878506DOA1
CSB-EP878506DOA1
CSB-BP878506DOA1
CSB-MP878506DOA1
CSB-EP878506DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative cysteine-rich repeat secretory protein 14 (CRRSP14) CSB-YP878507DOA
CSB-EP878507DOA
CSB-BP878507DOA
CSB-MP878507DOA
CSB-EP878507DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter B family member 19 (ABCB19), partial CSB-YP878508DOA
CSB-EP878508DOA
CSB-BP878508DOA
CSB-MP878508DOA
CSB-EP878508DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uridine kinase-like protein 2, chloroplastic (UKL2) CSB-YP878509DOA
CSB-EP878509DOA
CSB-BP878509DOA
CSB-MP878509DOA
CSB-EP878509DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor bHLH77 (BHLH77) CSB-YP878510DOA
CSB-EP878510DOA
CSB-BP878510DOA
CSB-MP878510DOA
CSB-EP878510DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g13150 (At3g13150), partial CSB-YP878511DOA
CSB-EP878511DOA
CSB-BP878511DOA
CSB-MP878511DOA
CSB-EP878511DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative DUF21 domain-containing protein At3g13070, chloroplastic (CBSDUFCH1), partial CSB-YP878512DOA1
CSB-EP878512DOA1
CSB-BP878512DOA1
CSB-MP878512DOA1
CSB-EP878512DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/kelch-repeat protein At3g27910 (At3g27910) CSB-YP878513DOA
CSB-EP878513DOA
CSB-BP878513DOA
CSB-MP878513DOA
CSB-EP878513DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable beta-1,3-galactosyltransferase 13 (B3GALT13), partial CSB-YP878514DOA1
CSB-EP878514DOA1
CSB-BP878514DOA1
CSB-MP878514DOA1
CSB-EP878514DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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