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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Zinc finger CCCH domain-containing protein 5 (At1g10320), partial CSB-YP871205DOA
CSB-EP871205DOA
CSB-BP871205DOA
CSB-MP871205DOA
CSB-EP871205DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 1-acyl-sn-glycerol-3-phosphate acyltransferase 3 (LPAT3), partial CSB-YP871206DOA1
CSB-EP871206DOA1
CSB-BP871206DOA1
CSB-MP871206DOA1
CSB-EP871206DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Aldehyde dehydrogenase family 7 member B4 (ALDH7B4), partial CSB-YP871207DOA
CSB-EP871207DOA
CSB-BP871207DOA
CSB-MP871207DOA
CSB-EP871207DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable glycerol-3-phosphate acyltransferase 3 (GPAT3), partial CSB-YP871208DOA1
CSB-EP871208DOA1
CSB-BP871208DOA1
CSB-MP871208DOA1
CSB-EP871208DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable salt tolerance-like protein At1g78600 (At1g78600) CSB-YP871209DOA
CSB-EP871209DOA
CSB-BP871209DOA
CSB-MP871209DOA
CSB-EP871209DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box/kelch-repeat protein At1g20940 (At1g20940) CSB-YP871210DOA
CSB-EP871210DOA
CSB-BP871210DOA
CSB-MP871210DOA
CSB-EP871210DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Dehydration-responsive element-binding protein 1C (DREB1C) CSB-YP871211DOA
CSB-EP871211DOA
CSB-BP871211DOA
CSB-MP871211DOA
CSB-EP871211DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 3-ketoacyl-CoA synthase 16 (KCS16), partial CSB-YP871212DOA1
CSB-EP871212DOA1
CSB-BP871212DOA1
CSB-MP871212DOA1
CSB-EP871212DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At4g26800 (At4g26800), partial CSB-YP871213DOA
CSB-EP871213DOA
CSB-BP871213DOA
CSB-MP871213DOA
CSB-EP871213DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative glutathione peroxidase 7, chloroplastic (GPX7) CSB-YP871214DOA
CSB-EP871214DOA
CSB-BP871214DOA
CSB-MP871214DOA
CSB-EP871214DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cullin-like protein 5 (At4g12100) CSB-YP871215DOA
CSB-EP871215DOA
CSB-BP871215DOA
CSB-MP871215DOA
CSB-EP871215DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative DEAD-box ATP-dependent RNA helicase 43 (RH43), partial CSB-YP871216DOA
CSB-EP871216DOA
CSB-BP871216DOA
CSB-MP871216DOA
CSB-EP871216DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Peroxidase 51 (PER51) CSB-YP871217DOA
CSB-EP871217DOA
CSB-BP871217DOA
CSB-MP871217DOA
CSB-EP871217DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana V-type proton ATPase subunit G3 (VHA-G3) CSB-YP871218DOA
CSB-EP871218DOA
CSB-BP871218DOA
CSB-MP871218DOA
CSB-EP871218DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana RING-H2 finger protein ATL18 (ATL18), partial CSB-YP871219DOA1
CSB-EP871219DOA1
CSB-BP871219DOA1
CSB-MP871219DOA1
CSB-EP871219DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At4g38150 (At4g38150) CSB-YP871220DOA
CSB-EP871220DOA
CSB-BP871220DOA
CSB-MP871220DOA
CSB-EP871220DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription repressor MYB4 (MYB4) CSB-YP871221DOA
CSB-EP871221DOA
CSB-BP871221DOA
CSB-MP871221DOA
CSB-EP871221DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Argininosuccinate synthase, chloroplastic (At4g24830) CSB-YP871222DOA
CSB-EP871222DOA
CSB-BP871222DOA
CSB-MP871222DOA
CSB-EP871222DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable protein phosphatase 2C 54 (At4g11040) CSB-YP871223DOA
CSB-EP871223DOA
CSB-BP871223DOA
CSB-MP871223DOA
CSB-EP871223DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/kelch-repeat protein At4g39240 (At4g39240) CSB-YP871224DOA
CSB-EP871224DOA
CSB-BP871224DOA
CSB-MP871224DOA
CSB-EP871224DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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