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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana Probable disease resistance protein At5g43730 (At5g43730), partial CSB-YP866917DOA
CSB-EP866917DOA
CSB-BP866917DOA
CSB-MP866917DOA
CSB-EP866917DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative B3 domain-containing protein At5g66980 (At5g66980) CSB-YP866918DOA
CSB-EP866918DOA
CSB-BP866918DOA
CSB-MP866918DOA
CSB-EP866918DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana E3 ubiquitin-protein ligase listerin (At5g58410), partial CSB-YP866919DOA
CSB-EP866919DOA
CSB-BP866919DOA
CSB-MP866919DOA
CSB-EP866919DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ras-related protein RABA5a (RABA5A) CSB-YP866920DOA
CSB-EP866920DOA
CSB-BP866920DOA
CSB-MP866920DOA
CSB-EP866920DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At5g51380 (At5g51380) CSB-YP866921DOA
CSB-EP866921DOA
CSB-BP866921DOA
CSB-MP866921DOA
CSB-EP866921DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cyclin-D3-2 (CYCD3-2) CSB-YP866922DOA
CSB-EP866922DOA
CSB-BP866922DOA
CSB-MP866922DOA
CSB-EP866922DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Two-component response regulator ARR18 (ARR18), partial CSB-YP866923DOA
CSB-EP866923DOA
CSB-BP866923DOA
CSB-MP866923DOA
CSB-EP866923DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable WRKY transcription factor 48 (WRKY48) CSB-YP866924DOA
CSB-EP866924DOA
CSB-BP866924DOA
CSB-MP866924DOA
CSB-EP866924DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor ILR3 (ILR3) CSB-YP866925DOA
CSB-EP866925DOA
CSB-BP866925DOA
CSB-MP866925DOA
CSB-EP866925DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Glutamate receptor 1.3 (GLR1.3), partial CSB-YP866926DOA
CSB-EP866926DOA
CSB-BP866926DOA
CSB-MP866926DOA
CSB-EP866926DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana DNA polymerase alpha catalytic subunit (POLA), partial CSB-YP866927DOA
CSB-EP866927DOA
CSB-BP866927DOA
CSB-MP866927DOA
CSB-EP866927DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cysteine-rich repeat secretory protein 57 (CRRSP57) CSB-YP866928DOA
CSB-EP866928DOA
CSB-BP866928DOA
CSB-MP866928DOA
CSB-EP866928DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 60S ribosomal protein L32-2 (RPL32B) CSB-YP866929DOA
CSB-EP866929DOA
CSB-BP866929DOA
CSB-MP866929DOA
CSB-EP866929DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein SKIP31 (SKIP31) CSB-YP866930DOA
CSB-EP866930DOA
CSB-BP866930DOA
CSB-MP866930DOA
CSB-EP866930DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable pectinesterase/pectinesterase inhibitor 59 (PME59), partial CSB-YP866931DOA
CSB-EP866931DOA
CSB-BP866931DOA
CSB-MP866931DOA
CSB-EP866931DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative mitochondrial outer membrane protein porin-like (At5g37610) CSB-YP866932DOA
CSB-EP866932DOA
CSB-BP866932DOA
CSB-MP866932DOA
CSB-EP866932DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana L-type lectin-domain containing receptor kinase S.6 (LECRKS6), partial CSB-YP866933DOA
CSB-EP866933DOA
CSB-BP866933DOA
CSB-MP866933DOA
CSB-EP866933DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative UDP-arabinose 4-epimerase 4 (At5g44480), partial CSB-YP866934DOA
CSB-EP866934DOA
CSB-BP866934DOA
CSB-MP866934DOA
CSB-EP866934DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At5g51000 (At5g51000) CSB-YP866935DOA
CSB-EP866935DOA
CSB-BP866935DOA
CSB-MP866935DOA
CSB-EP866935DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g48910 (PCMP-H38), partial CSB-YP866936DOA
CSB-EP866936DOA
CSB-BP866936DOA
CSB-MP866936DOA
CSB-EP866936DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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