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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Arabidopsis thaliana DEAD-box ATP-dependent RNA helicase 18 (RH18), partial CSB-YP863798DOA
CSB-EP863798DOA
CSB-BP863798DOA
CSB-MP863798DOA
CSB-EP863798DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Translation factor GUF1 homolog, mitochondrial (At5g39900) CSB-YP863799DOA
CSB-EP863799DOA
CSB-BP863799DOA
CSB-MP863799DOA
CSB-EP863799DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative nuclear matrix constituent protein 1-like protein (At5g65770), partial CSB-YP863800DOA
CSB-EP863800DOA
CSB-BP863800DOA
CSB-MP863800DOA
CSB-EP863800DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable receptor-like protein kinase At5g61350 (At5g61350), partial CSB-YP863801DOA
CSB-EP863801DOA
CSB-BP863801DOA
CSB-MP863801DOA
CSB-EP863801DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana 50S ribosomal protein L27, chloroplastic (RPL27) CSB-YP863802DOA
CSB-EP863802DOA
CSB-BP863802DOA
CSB-MP863802DOA
CSB-EP863802DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Formin-like protein 20 (FH20), partial CSB-YP863803DOA
CSB-EP863803DOA
CSB-BP863803DOA
CSB-MP863803DOA
CSB-EP863803DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ABC transporter A family member 9 (ABCA9), partial CSB-YP863804DOA
CSB-EP863804DOA
CSB-BP863804DOA
CSB-MP863804DOA
CSB-EP863804DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana B3 domain-containing protein At5g24050 (At5g24050) CSB-YP863805DOA
CSB-EP863805DOA
CSB-BP863805DOA
CSB-MP863805DOA
CSB-EP863805DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At5g52880 (At5g52880) CSB-YP863806DOA
CSB-EP863806DOA
CSB-BP863806DOA
CSB-MP863806DOA
CSB-EP863806DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At5g39460 (At5g39460), partial CSB-YP863807DOA
CSB-EP863807DOA
CSB-BP863807DOA
CSB-MP863807DOA
CSB-EP863807DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Hypersensitive-induced response protein 1 (HIR1) CSB-YP863808DOA
CSB-EP863808DOA
CSB-BP863808DOA
CSB-MP863808DOA
CSB-EP863808DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pectinesterase QRT1 (QRT1) CSB-YP863809DOA
CSB-EP863809DOA
CSB-BP863809DOA
CSB-MP863809DOA
CSB-EP863809DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box/FBD/LRR-repeat protein At5g56420 (At5g56420) CSB-YP863810DOA
CSB-EP863810DOA
CSB-BP863810DOA
CSB-MP863810DOA
CSB-EP863810DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative germin-like protein subfamily 1 member 12 (At5g38960) CSB-YP863811DOA
CSB-EP863811DOA
CSB-BP863811DOA
CSB-MP863811DOA
CSB-EP863811DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Transcription factor BIM3 (BIM3) CSB-YP863812DOA
CSB-EP863812DOA
CSB-BP863812DOA
CSB-MP863812DOA
CSB-EP863812DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g60960, mitochondrial (At5g60960) CSB-YP863813DOA
CSB-EP863813DOA
CSB-BP863813DOA
CSB-MP863813DOA
CSB-EP863813DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative cyclin-A3-1 (CYCA3-1) CSB-YP863814DOA
CSB-EP863814DOA
CSB-BP863814DOA
CSB-MP863814DOA
CSB-EP863814DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Protein LATERAL ORGAN BOUNDARIES (LOB) CSB-YP863815DOA
CSB-EP863815DOA
CSB-BP863815DOA
CSB-MP863815DOA
CSB-EP863815DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g12100, mitochondrial (At5g12100), partial CSB-YP863816DOA
CSB-EP863816DOA
CSB-BP863816DOA
CSB-MP863816DOA
CSB-EP863816DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana PHD finger protein MALE STERILITY 1 (MS1), partial CSB-YP863817DOA
CSB-EP863817DOA
CSB-BP863817DOA
CSB-MP863817DOA
CSB-EP863817DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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