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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Putative cysteine-rich repeat secretory protein 36 (CRRSP36) CSB-YP862855DOA
CSB-EP862855DOA
CSB-BP862855DOA
CSB-MP862855DOA
CSB-EP862855DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative cysteine-rich repeat secretory protein 31 (CRRSP31) CSB-YP862856DOA
CSB-EP862856DOA
CSB-BP862856DOA
CSB-MP862856DOA
CSB-EP862856DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative cysteine-rich repeat secretory protein 25 (CRRSP25) CSB-YP862857DOA
CSB-EP862857DOA
CSB-BP862857DOA
CSB-MP862857DOA
CSB-EP862857DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable pectate lyase 9 (At3g24230) CSB-YP862858DOA
CSB-EP862858DOA
CSB-BP862858DOA
CSB-MP862858DOA
CSB-EP862858DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative BTB/POZ domain-containing protein At3g29740 (At3g29740) CSB-YP862859DOA
CSB-EP862859DOA
CSB-BP862859DOA
CSB-MP862859DOA
CSB-EP862859DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Peroxisomal (S)-2-hydroxy-acid oxidase GLO1 (GLO1) CSB-YP862860DOA
CSB-EP862860DOA
CSB-BP862860DOA
CSB-MP862860DOA
CSB-EP862860DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Scarecrow-like protein 29 (SCL29), partial CSB-YP862861DOA
CSB-EP862861DOA
CSB-BP862861DOA
CSB-MP862861DOA
CSB-EP862861DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cyclin-dependent kinase inhibitor 5 (KRP5) CSB-YP862862DOA
CSB-EP862862DOA
CSB-BP862862DOA
CSB-MP862862DOA
CSB-EP862862DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Putative F-box protein At3g25750 (At3g25750) CSB-YP862863DOA
CSB-EP862863DOA
CSB-BP862863DOA
CSB-MP862863DOA
CSB-EP862863DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable S-adenosylmethionine-dependent methyltransferase At5g38100 (At5g38100) CSB-YP862864DOA
CSB-EP862864DOA
CSB-BP862864DOA
CSB-MP862864DOA
CSB-EP862864DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Aluminum-activated malate transporter 9 (ALMT9), partial CSB-YP862865DOA1
CSB-EP862865DOA1
CSB-BP862865DOA1
CSB-MP862865DOA1
CSB-EP862865DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At3g29230 (PCMP-E27), partial CSB-YP862866DOA
CSB-EP862866DOA
CSB-BP862866DOA
CSB-MP862866DOA
CSB-EP862866DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana RING-H2 finger protein ATL77 (ATL77), partial CSB-YP862867DOA1
CSB-EP862867DOA1
CSB-BP862867DOA1
CSB-MP862867DOA1
CSB-EP862867DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Membrane-anchored ubiquitin-fold protein 4 (MUB4) CSB-YP862868DOA
CSB-EP862868DOA
CSB-BP862868DOA
CSB-MP862868DOA
CSB-EP862868DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytochrome P450 82G1 (CYP82G1), partial CSB-YP862869DOA
CSB-EP862869DOA
CSB-BP862869DOA
CSB-MP862869DOA
CSB-EP862869DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At5g65660 (At5g65660), partial CSB-YP862870DOA1
CSB-EP862870DOA1
CSB-BP862870DOA1
CSB-MP862870DOA1
CSB-EP862870DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Pentatricopeptide repeat-containing protein At5g65560 (At5g65560), partial CSB-YP862871DOA
CSB-EP862871DOA
CSB-BP862871DOA
CSB-MP862871DOA
CSB-EP862871DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Chitinase-like protein 2 (CTL2) CSB-YP862872DOA
CSB-EP862872DOA
CSB-BP862872DOA
CSB-MP862872DOA
CSB-EP862872DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana L-type lectin-domain containing receptor kinase I.7 (LECRK17), partial CSB-YP862873DOA
CSB-EP862873DOA
CSB-BP862873DOA
CSB-MP862873DOA
CSB-EP862873DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana B3 domain-containing protein At5g60142 (At5g60142) CSB-YP862874DOA
CSB-EP862874DOA
CSB-BP862874DOA
CSB-MP862874DOA
CSB-EP862874DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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