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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bacillus halodurans 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP862555BQS
CSB-EP862555BQS
CSB-BP862555BQS
CSB-MP862555BQS
CSB-EP862555BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans UPF0060 membrane protein BH2744 (BH2744), partial CSB-YP862556BQS1
CSB-EP862556BQS1
CSB-BP862556BQS1
CSB-MP862556BQS1
CSB-EP862556BQS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Isocitrate lyase (aceA) CSB-YP862557BQS
CSB-EP862557BQS
CSB-BP862557BQS
CSB-MP862557BQS
CSB-EP862557BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans UPF0223 protein BH2638 (BH2638) CSB-YP862558BQS
CSB-EP862558BQS
CSB-BP862558BQS
CSB-MP862558BQS
CSB-EP862558BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Phosphopantetheine adenylyltransferase (coaD) CSB-YP862559BQS
CSB-EP862559BQS
CSB-BP862559BQS
CSB-MP862559BQS
CSB-EP862559BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Uncharacterized N-acetyltransferase BH2580 (BH2580) CSB-YP862560BQS
CSB-EP862560BQS
CSB-BP862560BQS
CSB-MP862560BQS
CSB-EP862560BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP862561BQS
CSB-EP862561BQS
CSB-BP862561BQS
CSB-MP862561BQS
CSB-EP862561BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Aspartate carbamoyltransferase (pyrB) CSB-YP862562BQS
CSB-EP862562BQS
CSB-BP862562BQS
CSB-MP862562BQS
CSB-EP862562BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Carbamoyl-phosphate synthase pyrimidine-specific large chain (pyrAB), partial CSB-YP862563BQS
CSB-EP862563BQS
CSB-BP862563BQS
CSB-MP862563BQS
CSB-EP862563BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP862564BQS
CSB-EP862564BQS
CSB-BP862564BQS
CSB-MP862564BQS
CSB-EP862564BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP862565BQS
CSB-EP862565BQS
CSB-BP862565BQS
CSB-MP862565BQS
CSB-EP862565BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans ATP-dependent protease ATPase subunit HslU (hslU) CSB-YP862566BQS
CSB-EP862566BQS
CSB-BP862566BQS
CSB-MP862566BQS
CSB-EP862566BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Protein RecA (recA) CSB-YP862567BQS
CSB-EP862567BQS
CSB-BP862567BQS
CSB-MP862567BQS
CSB-EP862567BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans UPF0154 protein BH2350 (BH2350), partial CSB-YP862568BQS
CSB-EP862568BQS
CSB-BP862568BQS
CSB-MP862568BQS
CSB-EP862568BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Small, acid-soluble spore protein P (sspP) CSB-YP862569BQS
CSB-EP862569BQS
CSB-BP862569BQS
CSB-MP862569BQS
CSB-EP862569BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Peptide methionine sulfoxide reductase MsrA (msrA) CSB-YP862570BQS
CSB-EP862570BQS
CSB-BP862570BQS
CSB-MP862570BQS
CSB-EP862570BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (kduI) CSB-YP862571BQS
CSB-EP862571BQS
CSB-BP862571BQS
CSB-MP862571BQS
CSB-EP862571BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Small, acid-soluble spore protein H (sspH) CSB-YP862572BQS
CSB-EP862572BQS
CSB-BP862572BQS
CSB-MP862572BQS
CSB-EP862572BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans Ribulokinase (araB), partial CSB-YP862573BQS
CSB-EP862573BQS
CSB-BP862573BQS
CSB-MP862573BQS
CSB-EP862573BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus halodurans UPF0398 protein BH1768 (BH1768) CSB-YP862574BQS
CSB-EP862574BQS
CSB-BP862574BQS
CSB-MP862574BQS
CSB-EP862574BQS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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