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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Caulobacter crescentus Diaminopimelate epimerase (dapF) CSB-YP860495DQO
CSB-EP860495DQO
CSB-BP860495DQO
CSB-MP860495DQO
CSB-EP860495DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Probable transaldolase (tal) CSB-YP860496DQO
CSB-EP860496DQO
CSB-BP860496DQO
CSB-MP860496DQO
CSB-EP860496DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Acetyl-coenzyme A carboxylase carboxyl transferase subunit beta (accD) CSB-YP860497DQO
CSB-EP860497DQO
CSB-BP860497DQO
CSB-MP860497DQO
CSB-EP860497DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus ATP synthase subunit beta (atpD), partial CSB-YP860498DQO
CSB-EP860498DQO
CSB-BP860498DQO
CSB-MP860498DQO
CSB-EP860498DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Ribonuclease HI (rnhA) CSB-YP860499DQO
CSB-EP860499DQO
CSB-BP860499DQO
CSB-MP860499DQO
CSB-EP860499DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus UPF0335 protein CC_3319 (CC_3319) CSB-YP860500DQO
CSB-EP860500DQO
CSB-BP860500DQO
CSB-MP860500DQO
CSB-EP860500DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Putative phosphoribosylaminoimidazole-succinocarboxamide synthase 2 (purC2) CSB-YP860501DQO
CSB-EP860501DQO
CSB-BP860501DQO
CSB-MP860501DQO
CSB-EP860501DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Protein imuB (imuB) CSB-YP860502DQO
CSB-EP860502DQO
CSB-BP860502DQO
CSB-MP860502DQO
CSB-EP860502DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus 7-carboxy-7-deazaguanine synthase (queE) CSB-YP860503DQO
CSB-EP860503DQO
CSB-BP860503DQO
CSB-MP860503DQO
CSB-EP860503DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Acetyl-coenzyme A carboxylase carboxyl transferase subunit alpha (accA) CSB-YP860504DQO
CSB-EP860504DQO
CSB-BP860504DQO
CSB-MP860504DQO
CSB-EP860504DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Putative aminoacrylate peracid reductase RutC (rutC) CSB-YP860505DQO
CSB-EP860505DQO
CSB-BP860505DQO
CSB-MP860505DQO
CSB-EP860505DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Methionine import ATP-binding protein MetN (metN) CSB-YP860506DQO
CSB-EP860506DQO
CSB-BP860506DQO
CSB-MP860506DQO
CSB-EP860506DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP860507DQO
CSB-EP860507DQO
CSB-BP860507DQO
CSB-MP860507DQO
CSB-EP860507DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Toxin RelE2 (relE2) CSB-YP860508DQO
CSB-EP860508DQO
CSB-BP860508DQO
CSB-MP860508DQO
CSB-EP860508DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Putative Holliday junction resolvase (CC_2439) CSB-YP860509DQO
CSB-EP860509DQO
CSB-BP860509DQO
CSB-MP860509DQO
CSB-EP860509DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus UPF0262 protein CC_2345 (CC_2345) CSB-YP860510DQO
CSB-EP860510DQO
CSB-BP860510DQO
CSB-MP860510DQO
CSB-EP860510DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Aspartate 1-decarboxylase (panD) CSB-YP860511DQO
CSB-EP860511DQO
CSB-BP860511DQO
CSB-MP860511DQO
CSB-EP860511DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP860512DQO
CSB-EP860512DQO
CSB-BP860512DQO
CSB-MP860512DQO
CSB-EP860512DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Sec-independent protein translocase protein TatB (tatB), partial CSB-YP860513DQO
CSB-EP860513DQO
CSB-BP860513DQO
CSB-MP860513DQO
CSB-EP860513DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus NADH-quinone oxidoreductase subunit C (nuoC) CSB-YP860514DQO
CSB-EP860514DQO
CSB-BP860514DQO
CSB-MP860514DQO
CSB-EP860514DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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