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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus D-alanine aminotransferase (dat) CSB-YP836852SUL
CSB-EP836852SUL
CSB-BP836852SUL
CSB-MP836852SUL
CSB-EP836852SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Septation ring formation regulator EzrA (ezrA), partial CSB-YP836853SUL
CSB-EP836853SUL
CSB-BP836853SUL
CSB-MP836853SUL
CSB-EP836853SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA polymerase III subunit alpha (dnaE), partial CSB-YP836854SUL
CSB-EP836854SUL
CSB-BP836854SUL
CSB-MP836854SUL
CSB-EP836854SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Probable GTP-binding protein EngB (engB) CSB-YP836855SUL
CSB-EP836855SUL
CSB-BP836855SUL
CSB-MP836855SUL
CSB-EP836855SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein grpE (grpE) CSB-YP836856SUL
CSB-EP836856SUL
CSB-BP836856SUL
CSB-MP836856SUL
CSB-EP836856SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribonuclease Z (rnz) CSB-YP836857SUL
CSB-EP836857SUL
CSB-BP836857SUL
CSB-MP836857SUL
CSB-EP836857SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 3-phosphoshikimate 1-carboxyvinyltransferase (aroA) CSB-YP836858SUL
CSB-EP836858SUL
CSB-BP836858SUL
CSB-MP836858SUL
CSB-EP836858SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Quinolone resistance protein norB (norB), partial CSB-YP836859SUL
CSB-EP836859SUL
CSB-BP836859SUL
CSB-MP836859SUL
CSB-EP836859SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (odhB) CSB-YP836860SUL
CSB-EP836860SUL
CSB-BP836860SUL
CSB-MP836860SUL
CSB-EP836860SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Conserved virulence factor B (cvfB) CSB-YP836861SUL
CSB-EP836861SUL
CSB-BP836861SUL
CSB-MP836861SUL
CSB-EP836861SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Nuclease sbcCD subunit D (sbcD) CSB-YP836862SUL
CSB-EP836862SUL
CSB-BP836862SUL
CSB-MP836862SUL
CSB-EP836862SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA pseudouridine synthase B (truB) CSB-YP836863SUL
CSB-EP836863SUL
CSB-BP836863SUL
CSB-MP836863SUL
CSB-EP836863SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribonuclease HII (rnhB) CSB-YP836864SUL
CSB-EP836864SUL
CSB-BP836864SUL
CSB-MP836864SUL
CSB-EP836864SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Guanylate kinase (gmk) CSB-YP836865SUL
CSB-EP836865SUL
CSB-BP836865SUL
CSB-MP836865SUL
CSB-EP836865SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP836866SUL
CSB-EP836866SUL
CSB-BP836866SUL
CSB-MP836866SUL
CSB-EP836866SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Heme-degrading monooxygenase isdG (isdG) CSB-YP836867SUL
CSB-EP836867SUL
CSB-BP836867SUL
CSB-MP836867SUL
CSB-EP836867SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Bifunctional purine biosynthesis protein PurH (purH) CSB-YP836868SUL
CSB-EP836868SUL
CSB-BP836868SUL
CSB-MP836868SUL
CSB-EP836868SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Bifunctional autolysin (atl), partial CSB-YP836869SUL
CSB-EP836869SUL
CSB-BP836869SUL
CSB-MP836869SUL
CSB-EP836869SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-lysine ligase (murE) CSB-YP836870SUL
CSB-EP836870SUL
CSB-BP836870SUL
CSB-MP836870SUL
CSB-EP836870SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP-dependent helicase/nuclease subunit A (addA), partial CSB-YP836871SUL
CSB-EP836871SUL
CSB-BP836871SUL
CSB-MP836871SUL
CSB-EP836871SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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