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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xanthomonas campestris pv. campestris LexA repressor 1 (lexA1) CSB-YP822992XAY
CSB-EP822992XAY
CSB-BP822992XAY
CSB-MP822992XAY
CSB-EP822992XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Molybdenum cofactor biosynthesis protein C (moaC) CSB-YP822993XAY
CSB-EP822993XAY
CSB-BP822993XAY
CSB-MP822993XAY
CSB-EP822993XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP822994XAY
CSB-EP822994XAY
CSB-BP822994XAY
CSB-MP822994XAY
CSB-EP822994XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 50S ribosomal protein L6 (rplF) CSB-YP822995XAY
CSB-EP822995XAY
CSB-BP822995XAY
CSB-MP822995XAY
CSB-EP822995XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 50S ribosomal protein L4 (rplD) CSB-YP822996XAY
CSB-EP822996XAY
CSB-BP822996XAY
CSB-MP822996XAY
CSB-EP822996XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 50S ribosomal protein L7/L12 (rplL) CSB-YP822997XAY
CSB-EP822997XAY
CSB-BP822997XAY
CSB-MP822997XAY
CSB-EP822997XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Peptide chain release factor 1 (prfA) CSB-YP822998XAY
CSB-EP822998XAY
CSB-BP822998XAY
CSB-MP822998XAY
CSB-EP822998XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Protein ApaG (apaG) CSB-YP822999XAY
CSB-EP822999XAY
CSB-BP822999XAY
CSB-MP822999XAY
CSB-EP822999XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Adenosylhomocysteinase (ahcY) CSB-YP823000XAY
CSB-EP823000XAY
CSB-BP823000XAY
CSB-MP823000XAY
CSB-EP823000XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP823001XAY
CSB-EP823001XAY
CSB-BP823001XAY
CSB-MP823001XAY
CSB-EP823001XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Potassium-transporting ATPase B chain (kdpB), partial CSB-YP823002XAY1
CSB-EP823002XAY1
CSB-BP823002XAY1
CSB-MP823002XAY1
CSB-EP823002XAY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Peptide deformylase 1 (def1) CSB-YP823003XAY
CSB-EP823003XAY
CSB-BP823003XAY
CSB-MP823003XAY
CSB-EP823003XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris ATP synthase epsilon chain (atpC) CSB-YP823004XAY
CSB-EP823004XAY
CSB-BP823004XAY
CSB-MP823004XAY
CSB-EP823004XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 3-dehydroquinate dehydratase (aroQ) CSB-YP823005XAY
CSB-EP823005XAY
CSB-BP823005XAY
CSB-MP823005XAY
CSB-EP823005XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Probable lipid kinase YegS-like (XCC0458) CSB-YP823006XAY
CSB-EP823006XAY
CSB-BP823006XAY
CSB-MP823006XAY
CSB-EP823006XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris Pimelyl-[acyl-carrier protein] methyl ester esterase (bioH) CSB-YP823007XAY
CSB-EP823007XAY
CSB-BP823007XAY
CSB-MP823007XAY
CSB-EP823007XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas campestris pv. campestris 1,4-alpha-glucan branching enzyme GlgB 1 (glgB1), partial CSB-YP823008XAY
CSB-EP823008XAY
CSB-BP823008XAY
CSB-MP823008XAY
CSB-EP823008XAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas axonopodis pv. citri UPF0225 protein XAC4297 (XAC4297) CSB-YP823009XAD
CSB-EP823009XAD
CSB-BP823009XAD
CSB-MP823009XAD
CSB-EP823009XAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas axonopodis pv. citri Recombination-associated protein RdgC (rdgC) CSB-YP823010XAD
CSB-EP823010XAD
CSB-BP823010XAD
CSB-MP823010XAD
CSB-EP823010XAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas axonopodis pv. citri UPF0745 protein XAC4085 (XAC4085) CSB-YP823011XAD
CSB-EP823011XAD
CSB-BP823011XAD
CSB-MP823011XAD
CSB-EP823011XAD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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