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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Corynebacterium glutamicum Dihydroxy-acid dehydratase (ilvD), partial CSB-YP818860DWY
CSB-EP818860DWY
CSB-BP818860DWY
CSB-MP818860DWY
CSB-EP818860DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP818861DWY
CSB-EP818861DWY
CSB-BP818861DWY
CSB-MP818861DWY
CSB-EP818861DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP818862DWY
CSB-EP818862DWY
CSB-BP818862DWY
CSB-MP818862DWY
CSB-EP818862DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Ribose-phosphate pyrophosphokinase (prs) CSB-YP818863DWY
CSB-EP818863DWY
CSB-BP818863DWY
CSB-MP818863DWY
CSB-EP818863DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 50S ribosomal protein L31 type B (rpmE2) CSB-YP818864DWY
CSB-EP818864DWY
CSB-BP818864DWY
CSB-MP818864DWY
CSB-EP818864DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Thymidylate synthase (thyA) CSB-YP818865DWY
CSB-EP818865DWY
CSB-BP818865DWY
CSB-MP818865DWY
CSB-EP818865DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Probable potassium transport system protein kup (kup), partial CSB-YP818866DWY
CSB-EP818866DWY
CSB-BP818866DWY
CSB-MP818866DWY
CSB-EP818866DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Tryptophan--tRNA ligase (trpS) CSB-YP818867DWY
CSB-EP818867DWY
CSB-BP818867DWY
CSB-MP818867DWY
CSB-EP818867DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum NAD-dependent protein deacylase (cobB) CSB-YP818868DWY
CSB-EP818868DWY
CSB-BP818868DWY
CSB-MP818868DWY
CSB-EP818868DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 30S ribosomal protein S9 (rpsI) CSB-YP818869DWY
CSB-EP818869DWY
CSB-BP818869DWY
CSB-MP818869DWY
CSB-EP818869DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 30S ribosomal protein S5 (rpsE) CSB-YP818870DWY
CSB-EP818870DWY
CSB-BP818870DWY
CSB-MP818870DWY
CSB-EP818870DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 50S ribosomal protein L14 (rplN) CSB-YP818871DWY
CSB-EP818871DWY
CSB-BP818871DWY
CSB-MP818871DWY
CSB-EP818871DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Elongation factor G (fusA), partial CSB-YP818872DWY
CSB-EP818872DWY
CSB-BP818872DWY
CSB-MP818872DWY
CSB-EP818872DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Demethylmenaquinone methyltransferase (ubiE) CSB-YP818873DWY
CSB-EP818873DWY
CSB-BP818873DWY
CSB-MP818873DWY
CSB-EP818873DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA) CSB-YP818874DWY
CSB-EP818874DWY
CSB-BP818874DWY
CSB-MP818874DWY
CSB-EP818874DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum Glutamyl-Q tRNA (Asp) synthetase (gluQ) CSB-YP818875DWY
CSB-EP818875DWY
CSB-BP818875DWY
CSB-MP818875DWY
CSB-EP818875DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Corynebacterium glutamicum UPF0232 protein Cgl0005/cg0006 (Cgl0005, cg0006) CSB-YP818876DWY
CSB-EP818876DWY
CSB-BP818876DWY
CSB-MP818876DWY
CSB-EP818876DWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative ABC transporter ATP-binding protein MW2603 (MW2603), partial CSB-YP818877SUL
CSB-EP818877SUL
CSB-BP818877SUL
CSB-MP818877SUL
CSB-EP818877SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine-rich adhesin for platelets (sraP), partial CSB-YP818878SUL
CSB-EP818878SUL
CSB-BP818878SUL
CSB-MP818878SUL
CSB-EP818878SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus HTH-type transcriptional regulator ArcR (arcR) CSB-YP818879SUL
CSB-EP818879SUL
CSB-BP818879SUL
CSB-MP818879SUL
CSB-EP818879SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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