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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Arabidopsis thaliana Two-component response regulator ARR14 (ARR14) CSB-YP814481DOA
CSB-EP814481DOA
CSB-BP814481DOA
CSB-MP814481DOA
CSB-EP814481DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Methyl-CpG-binding domain-containing protein 2 (MBD2) CSB-YP814482DOA
CSB-EP814482DOA
CSB-BP814482DOA
CSB-MP814482DOA
CSB-EP814482DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable uridine nucleosidase 2 (URH2) CSB-YP814483DOA
CSB-EP814483DOA
CSB-BP814483DOA
CSB-MP814483DOA
CSB-EP814483DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Succinyl-CoA ligase [ADP-forming] subunit alpha-2, mitochondrial (At5g23250) CSB-YP814484DOA
CSB-EP814484DOA
CSB-BP814484DOA
CSB-MP814484DOA
CSB-EP814484DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Nucleoside diphosphate kinase IV, chloroplastic/mitochondrial (NDK4) CSB-YP814485DOA
CSB-EP814485DOA
CSB-BP814485DOA
CSB-MP814485DOA
CSB-EP814485DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Auxin-responsive protein IAA26 (IAA26) CSB-YP814486DOA
CSB-EP814486DOA
CSB-BP814486DOA
CSB-MP814486DOA
CSB-EP814486DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana S-formylglutathione hydrolase (SFGH) CSB-YP814487DOA
CSB-EP814487DOA
CSB-BP814487DOA
CSB-MP814487DOA
CSB-EP814487DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Dihydrodipicolinate reductase 2, chloroplastic (DAPB2) CSB-YP814488DOA
CSB-EP814488DOA
CSB-BP814488DOA
CSB-MP814488DOA
CSB-EP814488DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana ADP,ATP carrier protein ER-ANT1 (ER-ANT1), partial CSB-YP814489DOA1
CSB-EP814489DOA1
CSB-BP814489DOA1
CSB-MP814489DOA1
CSB-EP814489DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Uncharacterized protein At3g58460 (At3g58460), partial CSB-YP814490DOA1
CSB-EP814490DOA1
CSB-BP814490DOA1
CSB-MP814490DOA1
CSB-EP814490DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana F-box protein At3g58530 (At3g58530) CSB-YP814491DOA
CSB-EP814491DOA
CSB-BP814491DOA
CSB-MP814491DOA
CSB-EP814491DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Peptidyl-prolyl cis-trans isomerase FKBP17-3, chloroplastic (FKBP17-3) CSB-YP814492DOA
CSB-EP814492DOA
CSB-BP814492DOA
CSB-MP814492DOA
CSB-EP814492DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Sugar transporter ERD6-like 16 (At5g18840), partial CSB-YP814493DOA
CSB-EP814493DOA
CSB-BP814493DOA
CSB-MP814493DOA
CSB-EP814493DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Calcium-activated outward-rectifying potassium channel 1 (KCO1), partial CSB-YP814494DOA1
CSB-EP814494DOA1
CSB-BP814494DOA1
CSB-MP814494DOA1
CSB-EP814494DOA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Ethylene-responsive transcription factor ERF034 (ERF034) CSB-YP814495DOA
CSB-EP814495DOA
CSB-BP814495DOA
CSB-MP814495DOA
CSB-EP814495DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Monothiol glutaredoxin-S12, chloroplastic (GRXS12) CSB-YP814496DOA
CSB-EP814496DOA
CSB-BP814496DOA
CSB-MP814496DOA
CSB-EP814496DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana OTU domain-containing protein At3g57810 (At3g57810) CSB-YP814497DOA
CSB-EP814497DOA
CSB-BP814497DOA
CSB-MP814497DOA
CSB-EP814497DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Succinate dehydrogenase [ubiquinone] iron-sulfur subunit 1, mitochondrial (SDH2-1) CSB-YP814498DOA
CSB-EP814498DOA
CSB-BP814498DOA
CSB-MP814498DOA
CSB-EP814498DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Probable xyloglucan endotransglucosylase/hydrolase protein 33 (XTH33) CSB-YP814499DOA
CSB-EP814499DOA
CSB-BP814499DOA
CSB-MP814499DOA
CSB-EP814499DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana GATA transcription factor 18 (GATA18) CSB-YP814500DOA
CSB-EP814500DOA
CSB-BP814500DOA
CSB-MP814500DOA
CSB-EP814500DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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