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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bradyrhizobium japonicum ATP synthase epsilon chain (atpC) CSB-YP805782BVW
CSB-EP805782BVW
CSB-BP805782BVW
CSB-MP805782BVW
CSB-EP805782BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 3-isopropylmalate dehydratase large subunit 1 (leuC1) CSB-YP805783BVW
CSB-EP805783BVW
CSB-BP805783BVW
CSB-MP805783BVW
CSB-EP805783BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Ribosomal RNA large subunit methyltransferase F (rlmF) CSB-YP805784BDU
CSB-EP805784BDU
CSB-BP805784BDU
CSB-MP805784BDU
CSB-EP805784BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Dephospho-CoA kinase (coaE) CSB-YP805785BDU
CSB-EP805785BDU
CSB-BP805785BDU
CSB-MP805785BDU
CSB-EP805785BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron tRNA modification GTPase MnmE (mnmE) CSB-YP805786BDU
CSB-EP805786BDU
CSB-BP805786BDU
CSB-MP805786BDU
CSB-EP805786BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Adenylate kinase (adk) CSB-YP805787BDU
CSB-EP805787BDU
CSB-BP805787BDU
CSB-MP805787BDU
CSB-EP805787BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 50S ribosomal protein L21 (rplU) CSB-YP805788BDU
CSB-EP805788BDU
CSB-BP805788BDU
CSB-MP805788BDU
CSB-EP805788BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron DNA replication and repair protein recF (recF) CSB-YP805789BDU
CSB-EP805789BDU
CSB-BP805789BDU
CSB-MP805789BDU
CSB-EP805789BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) CSB-YP805790BDU
CSB-EP805790BDU
CSB-BP805790BDU
CSB-MP805790BDU
CSB-EP805790BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron tRNA dimethylallyltransferase 1 (miaA1) CSB-YP805791BDU
CSB-EP805791BDU
CSB-BP805791BDU
CSB-MP805791BDU
CSB-EP805791BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP805792BDU
CSB-EP805792BDU
CSB-BP805792BDU
CSB-MP805792BDU
CSB-EP805792BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD) CSB-YP805793BDU
CSB-EP805793BDU
CSB-BP805793BDU
CSB-MP805793BDU
CSB-EP805793BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP805794BDU
CSB-EP805794BDU
CSB-BP805794BDU
CSB-MP805794BDU
CSB-EP805794BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Release factor glutamine methyltransferase (prmC) CSB-YP805795BDU
CSB-EP805795BDU
CSB-BP805795BDU
CSB-MP805795BDU
CSB-EP805795BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron 50S ribosomal protein L34 (rpmH) CSB-YP805796BDU
CSB-EP805796BDU
CSB-BP805796BDU
CSB-MP805796BDU
CSB-EP805796BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Protein MraZ (mraZ) CSB-YP805797BDU
CSB-EP805797BDU
CSB-BP805797BDU
CSB-MP805797BDU
CSB-EP805797BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP805798BDU
CSB-EP805798BDU
CSB-BP805798BDU
CSB-MP805798BDU
CSB-EP805798BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Shikimate kinase (aroK) CSB-YP805799BDU
CSB-EP805799BDU
CSB-BP805799BDU
CSB-MP805799BDU
CSB-EP805799BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG), partial CSB-YP805800BDU
CSB-EP805800BDU
CSB-BP805800BDU
CSB-MP805800BDU
CSB-EP805800BDU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacteroides thetaiotaomicron Undecaprenyl-diphosphatase (uppP), partial CSB-YP805801BDU1
CSB-EP805801BDU1
CSB-BP805801BDU1
CSB-MP805801BDU1
CSB-EP805801BDU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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