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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Elongation factor Tu (tufA) CSB-YP803183CMN
CSB-EP803183CMN
CSB-BP803183CMN
CSB-MP803183CMN
CSB-EP803183CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Ribosomal RNA large subunit methyltransferase L (rlmL), partial CSB-YP803184XAT
CSB-EP803184XAT
CSB-BP803184XAT
CSB-MP803184XAT
CSB-EP803184XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Peptidyl-tRNA hydrolase (pth) CSB-YP803185XAT
CSB-EP803185XAT
CSB-BP803185XAT
CSB-MP803185XAT
CSB-EP803185XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa DNA-directed RNA polymerase subunit beta (rpoB), partial CSB-YP803186XAT
CSB-EP803186XAT
CSB-BP803186XAT
CSB-MP803186XAT
CSB-EP803186XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UPF0176 protein PD_1985 (PD_1985) CSB-YP803187XAT
CSB-EP803187XAT
CSB-BP803187XAT
CSB-MP803187XAT
CSB-EP803187XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Asparagine--tRNA ligase (asnS) CSB-YP803188XAT
CSB-EP803188XAT
CSB-BP803188XAT
CSB-MP803188XAT
CSB-EP803188XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP803189XAT
CSB-EP803189XAT
CSB-BP803189XAT
CSB-MP803189XAT
CSB-EP803189XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP803190XAT
CSB-EP803190XAT
CSB-BP803190XAT
CSB-MP803190XAT
CSB-EP803190XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UPF0394 membrane protein PD_1892 (PD_1892), partial CSB-YP803191XAT1
CSB-EP803191XAT1
CSB-BP803191XAT1
CSB-MP803191XAT1
CSB-EP803191XAT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) CSB-YP803192XAT
CSB-EP803192XAT
CSB-BP803192XAT
CSB-MP803192XAT
CSB-EP803192XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Phosphoglycerate kinase (pgk) CSB-YP803193XAT
CSB-EP803193XAT
CSB-BP803193XAT
CSB-MP803193XAT
CSB-EP803193XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Protein-L-isoaspartate O-methyltransferase (pcm) CSB-YP803194XAT
CSB-EP803194XAT
CSB-BP803194XAT
CSB-MP803194XAT
CSB-EP803194XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP803195XAT
CSB-EP803195XAT
CSB-BP803195XAT
CSB-MP803195XAT
CSB-EP803195XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Probable malate:quinone oxidoreductase (mqo), partial CSB-YP803196XAT
CSB-EP803196XAT
CSB-BP803196XAT
CSB-MP803196XAT
CSB-EP803196XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Dihydrodipicolinate synthase (dapA) CSB-YP803197XAT
CSB-EP803197XAT
CSB-BP803197XAT
CSB-MP803197XAT
CSB-EP803197XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa tRNA-dihydrouridine synthase A (dusA) CSB-YP803198XAT
CSB-EP803198XAT
CSB-BP803198XAT
CSB-MP803198XAT
CSB-EP803198XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 30S ribosomal protein S21 (rpsU) CSB-YP803199XAT
CSB-EP803199XAT
CSB-BP803199XAT
CSB-MP803199XAT
CSB-EP803199XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UPF0102 protein PD_1586 (PD_1586) CSB-YP803200XAT
CSB-EP803200XAT
CSB-BP803200XAT
CSB-MP803200XAT
CSB-EP803200XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Recombination-associated protein RdgC (rdgC) CSB-YP803201XAT
CSB-EP803201XAT
CSB-BP803201XAT
CSB-MP803201XAT
CSB-EP803201XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 60 kDa chaperonin (groL), partial CSB-YP803202XAT
CSB-EP803202XAT
CSB-BP803202XAT
CSB-MP803202XAT
CSB-EP803202XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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