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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xylella fastidiosa Thiazole synthase (thiG) CSB-YP771510XAT
CSB-EP771510XAT
CSB-BP771510XAT
CSB-MP771510XAT
CSB-EP771510XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (murG) CSB-YP771511XAT
CSB-EP771511XAT
CSB-BP771511XAT
CSB-MP771511XAT
CSB-EP771511XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa LPS-assembly protein lptD (lptD), partial CSB-YP771512XAT
CSB-EP771512XAT
CSB-BP771512XAT
CSB-MP771512XAT
CSB-EP771512XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 5'-nucleotidase surE (surE) CSB-YP771513XAT
CSB-EP771513XAT
CSB-BP771513XAT
CSB-MP771513XAT
CSB-EP771513XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Ribosomal RNA small subunit methyltransferase B (rsmB) CSB-YP771514XAT
CSB-EP771514XAT
CSB-BP771514XAT
CSB-MP771514XAT
CSB-EP771514XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Transcriptional repressor NrdR (nrdR) CSB-YP771515XAT
CSB-EP771515XAT
CSB-BP771515XAT
CSB-MP771515XAT
CSB-EP771515XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa UvrABC system protein B (uvrB), partial CSB-YP771516XAT
CSB-EP771516XAT
CSB-BP771516XAT
CSB-MP771516XAT
CSB-EP771516XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Putative cytochrome P450 133B1 (CYP133B1) CSB-YP771517XAT
CSB-EP771517XAT
CSB-BP771517XAT
CSB-MP771517XAT
CSB-EP771517XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Proline--tRNA ligase (proS), partial CSB-YP771518XAT
CSB-EP771518XAT
CSB-BP771518XAT
CSB-MP771518XAT
CSB-EP771518XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Ribosomal RNA small subunit methyltransferase I (rsmI) CSB-YP771519XAT
CSB-EP771519XAT
CSB-BP771519XAT
CSB-MP771519XAT
CSB-EP771519XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (fabA) CSB-YP771520XAT
CSB-EP771520XAT
CSB-BP771520XAT
CSB-MP771520XAT
CSB-EP771520XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Exodeoxyribonuclease 7 small subunit (xseB) CSB-YP771521XAT
CSB-EP771521XAT
CSB-BP771521XAT
CSB-MP771521XAT
CSB-EP771521XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Cytochrome c-type biogenesis protein CcmE (ccmE), partial CSB-YP771522XAT
CSB-EP771522XAT
CSB-BP771522XAT
CSB-MP771522XAT
CSB-EP771522XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP771523XAT
CSB-EP771523XAT
CSB-BP771523XAT
CSB-MP771523XAT
CSB-EP771523XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Lipoprotein signal peptidase (lspA), partial CSB-YP771524XAT1
CSB-EP771524XAT1
CSB-BP771524XAT1
CSB-MP771524XAT1
CSB-EP771524XAT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 23S rRNA (uracil (1939)-C (5))-methyltransferase RlmD (rlmD) CSB-YP771525XAT
CSB-EP771525XAT
CSB-BP771525XAT
CSB-MP771525XAT
CSB-EP771525XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Chaperone protein DnaJ (dnaJ) CSB-YP771526XAT
CSB-EP771526XAT
CSB-BP771526XAT
CSB-MP771526XAT
CSB-EP771526XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa Uncharacterized protein PD_1345 (PD_1345) CSB-YP771527XAT
CSB-EP771527XAT
CSB-BP771527XAT
CSB-MP771527XAT
CSB-EP771527XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (metE), partial CSB-YP771528XAT
CSB-EP771528XAT
CSB-BP771528XAT
CSB-MP771528XAT
CSB-EP771528XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa DNA repair protein recO (recO) CSB-YP771529XAT
CSB-EP771529XAT
CSB-BP771529XAT
CSB-MP771529XAT
CSB-EP771529XAT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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