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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Bacillus thuringiensis subsp. konkukian Ferredoxin--NADP reductase 2 (BT9727_4639) CSB-YP757017BAAE
CSB-EP757017BAAE
CSB-BP757017BAAE
CSB-MP757017BAAE
CSB-EP757017BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Putative membrane protein insertion efficiency factor (BT9727_4526) CSB-YP757018BAAE
CSB-EP757018BAAE
CSB-BP757018BAAE
CSB-MP757018BAAE
CSB-EP757018BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Tyrosine--tRNA ligase 1 (tyrS1) CSB-YP757019BAAE
CSB-EP757019BAAE
CSB-BP757019BAAE
CSB-MP757019BAAE
CSB-EP757019BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Acetate kinase (ackA) CSB-YP757020BAAE
CSB-EP757020BAAE
CSB-BP757020BAAE
CSB-MP757020BAAE
CSB-EP757020BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Dephospho-CoA kinase (coaE) CSB-YP757021BAAE
CSB-EP757021BAAE
CSB-BP757021BAAE
CSB-MP757021BAAE
CSB-EP757021BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Non-canonical purine NTP pyrophosphatase (BT9727_4217) CSB-YP757022BAAE
CSB-EP757022BAAE
CSB-BP757022BAAE
CSB-MP757022BAAE
CSB-EP757022BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP757023BAAE
CSB-EP757023BAAE
CSB-BP757023BAAE
CSB-MP757023BAAE
CSB-EP757023BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Aspartate--tRNA ligase (aspS), partial CSB-YP757024BAAE
CSB-EP757024BAAE
CSB-BP757024BAAE
CSB-MP757024BAAE
CSB-EP757024BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Transcription elongation factor GreA (greA) CSB-YP757025BAAE
CSB-EP757025BAAE
CSB-BP757025BAAE
CSB-MP757025BAAE
CSB-EP757025BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Shikimate dehydrogenase (aroE) CSB-YP757026BAAE
CSB-EP757026BAAE
CSB-BP757026BAAE
CSB-MP757026BAAE
CSB-EP757026BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Heat-inducible transcription repressor HrcA (hrcA) CSB-YP757027BAAE
CSB-EP757027BAAE
CSB-BP757027BAAE
CSB-MP757027BAAE
CSB-EP757027BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian DNA repair protein recO (recO) CSB-YP757028BAAE
CSB-EP757028BAAE
CSB-BP757028BAAE
CSB-MP757028BAAE
CSB-EP757028BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase 2 (murG2) CSB-YP757029BAAE
CSB-EP757029BAAE
CSB-BP757029BAAE
CSB-MP757029BAAE
CSB-EP757029BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Elongation factor P (efp) CSB-YP757030BAAE
CSB-EP757030BAAE
CSB-BP757030BAAE
CSB-MP757030BAAE
CSB-EP757030BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Acetylglutamate kinase (argB) CSB-YP757031BAAE
CSB-EP757031BAAE
CSB-BP757031BAAE
CSB-MP757031BAAE
CSB-EP757031BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Segregation and condensation protein A (scpA) CSB-YP757032BAAE
CSB-EP757032BAAE
CSB-BP757032BAAE
CSB-MP757032BAAE
CSB-EP757032BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX) CSB-YP757033BAAE
CSB-EP757033BAAE
CSB-BP757033BAAE
CSB-MP757033BAAE
CSB-EP757033BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) CSB-YP757034BAAE
CSB-EP757034BAAE
CSB-BP757034BAAE
CSB-MP757034BAAE
CSB-EP757034BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP757035BAAE
CSB-EP757035BAAE
CSB-BP757035BAAE
CSB-MP757035BAAE
CSB-EP757035BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus thuringiensis subsp. konkukian Carbamoyl-phosphate synthase large chain (carB), partial CSB-YP757036BAAE
CSB-EP757036BAAE
CSB-BP757036BAAE
CSB-MP757036BAAE
CSB-EP757036BAAE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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