重組蛋白

重組蛋白是解析生物相互作用的關(guān)鍵工具,廣泛應(yīng)用于各類研究實(shí)驗(yàn)中。無論是商業(yè)化產(chǎn)品,還是為特定研究定制的蛋白,它們都在多種科學(xué)應(yīng)用中發(fā)揮著不可替代的作用。
若您需要優(yōu)質(zhì)重組蛋白——已驗(yàn)證的生物活性、低內(nèi)毒素水平、高純度——來為您的研究提供可靠支持,這里正是您的理想之選。
歷經(jīng)18年積累,華美生物已構(gòu)建覆蓋原核與真核的五大蛋白質(zhì)表達(dá)系統(tǒng)。我們擁有包括桿狀病毒外膜蛋白(OMP)表達(dá)技術(shù)、膜蛋白表達(dá)技術(shù)等在內(nèi)的多項(xiàng)蛋白表達(dá)與純化專有技術(shù),致力于為您提供高質(zhì)量的重組蛋白解決方案。
產(chǎn)品特點(diǎn)
- 覆蓋廣泛:擁有超過16,000種重組蛋白,涵蓋人類、小鼠、大鼠、兔、豚鼠、雞、猴、豬等多種物種的基因與靶點(diǎn),支持跨物種比較研究。
- 表達(dá)系統(tǒng)全面:提供五大表達(dá)系統(tǒng),包括大腸桿菌、酵母、桿狀病毒-昆蟲細(xì)胞、哺乳動(dòng)物細(xì)胞以及特有的無細(xì)胞表達(dá)系統(tǒng)。該系統(tǒng)省去質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、破碎離心等步驟,顯著提升蛋白制備效率。
- 多標(biāo)簽選擇:支持His、GST、Flag、MBP等多種標(biāo)簽,便于后續(xù)純化、檢測與功能研究。
- 多領(lǐng)域應(yīng)用:適用于抗原制備、細(xì)胞與分析、蛋白互作、藥物研發(fā)、酶活性檢測、ELISA標(biāo)準(zhǔn)品、體內(nèi)外研究、質(zhì)譜與結(jié)構(gòu)分析等廣泛場景。
- 無動(dòng)物源成分:我們已建立全程無動(dòng)物副產(chǎn)品接觸的重組蛋白生產(chǎn)線,確保產(chǎn)品的高純凈性與一致性,適用于對(duì)來源敏感的各類實(shí)驗(yàn)。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì),或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的重組蛋白,請查看華美生物蛋白定制服務(wù)。
通過選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
| 產(chǎn)品名稱 | 貨號(hào) | 規(guī)格 |
|---|---|---|
| Recombinant Bacillus thuringiensis subsp. konkukian Ferredoxin--NADP reductase 2 (BT9727_4639) | CSB-YP757017BAAE CSB-EP757017BAAE CSB-BP757017BAAE CSB-MP757017BAAE CSB-EP757017BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian Putative membrane protein insertion efficiency factor (BT9727_4526) | CSB-YP757018BAAE CSB-EP757018BAAE CSB-BP757018BAAE CSB-MP757018BAAE CSB-EP757018BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian Tyrosine--tRNA ligase 1 (tyrS1) | CSB-YP757019BAAE CSB-EP757019BAAE CSB-BP757019BAAE CSB-MP757019BAAE CSB-EP757019BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian Acetate kinase (ackA) | CSB-YP757020BAAE CSB-EP757020BAAE CSB-BP757020BAAE CSB-MP757020BAAE CSB-EP757020BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian Dephospho-CoA kinase (coaE) | CSB-YP757021BAAE CSB-EP757021BAAE CSB-BP757021BAAE CSB-MP757021BAAE CSB-EP757021BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian Non-canonical purine NTP pyrophosphatase (BT9727_4217) | CSB-YP757022BAAE CSB-EP757022BAAE CSB-BP757022BAAE CSB-MP757022BAAE CSB-EP757022BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian Holliday junction ATP-dependent DNA helicase RuvB (ruvB) | CSB-YP757023BAAE CSB-EP757023BAAE CSB-BP757023BAAE CSB-MP757023BAAE CSB-EP757023BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian Aspartate--tRNA ligase (aspS), partial | CSB-YP757024BAAE CSB-EP757024BAAE CSB-BP757024BAAE CSB-MP757024BAAE CSB-EP757024BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian Transcription elongation factor GreA (greA) | CSB-YP757025BAAE CSB-EP757025BAAE CSB-BP757025BAAE CSB-MP757025BAAE CSB-EP757025BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian Shikimate dehydrogenase (aroE) | CSB-YP757026BAAE CSB-EP757026BAAE CSB-BP757026BAAE CSB-MP757026BAAE CSB-EP757026BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian Heat-inducible transcription repressor HrcA (hrcA) | CSB-YP757027BAAE CSB-EP757027BAAE CSB-BP757027BAAE CSB-MP757027BAAE CSB-EP757027BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian DNA repair protein recO (recO) | CSB-YP757028BAAE CSB-EP757028BAAE CSB-BP757028BAAE CSB-MP757028BAAE CSB-EP757028BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase 2 (murG2) | CSB-YP757029BAAE CSB-EP757029BAAE CSB-BP757029BAAE CSB-MP757029BAAE CSB-EP757029BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian Elongation factor P (efp) | CSB-YP757030BAAE CSB-EP757030BAAE CSB-BP757030BAAE CSB-MP757030BAAE CSB-EP757030BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian Acetylglutamate kinase (argB) | CSB-YP757031BAAE CSB-EP757031BAAE CSB-BP757031BAAE CSB-MP757031BAAE CSB-EP757031BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian Segregation and condensation protein A (scpA) | CSB-YP757032BAAE CSB-EP757032BAAE CSB-BP757032BAAE CSB-MP757032BAAE CSB-EP757032BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX) | CSB-YP757033BAAE CSB-EP757033BAAE CSB-BP757033BAAE CSB-MP757033BAAE CSB-EP757033BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase (dapH) | CSB-YP757034BAAE CSB-EP757034BAAE CSB-BP757034BAAE CSB-MP757034BAAE CSB-EP757034BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian Ribosomal RNA small subunit methyltransferase H (rsmH) | CSB-YP757035BAAE CSB-EP757035BAAE CSB-BP757035BAAE CSB-MP757035BAAE CSB-EP757035BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Bacillus thuringiensis subsp. konkukian Carbamoyl-phosphate synthase large chain (carB), partial | CSB-YP757036BAAE CSB-EP757036BAAE CSB-BP757036BAAE CSB-MP757036BAAE CSB-EP757036BAAE-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。
第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條:如何選擇合適的表達(dá)載體?
表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。
根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022






