重組蛋白
重組蛋白是解析生物相互作用的關(guān)鍵工具，廣泛應(yīng)用于各類研究實驗中。無論是商業(yè)化產(chǎn)品，還是為特定研究定制的蛋白，它們都在多種科學(xué)應(yīng)用中發(fā)揮著不可替代的作用。
若您需要優(yōu)質(zhì)重組蛋白——已驗證的生物活性、低內(nèi)毒素水平、高純度——來為您的研究提供可靠支持，這里正是您的理想之選。
歷經(jīng)18年積累，華美生物已構(gòu)建覆蓋原核與真核的五大蛋白質(zhì)表達系統(tǒng)。我們擁有包括桿狀病毒外膜蛋白（OMP）表達技術(shù)、膜蛋白表達技術(shù)等在內(nèi)的多項蛋白表達與純化專有技術(shù)，致力于為您提供高質(zhì)量的重組蛋白解決方案。
產(chǎn)品特點
- 覆蓋廣泛：擁有超過16,000種重組蛋白，涵蓋人類、小鼠、大鼠、兔、豚鼠、雞、猴、豬等多種物種的基因與靶點，支持跨物種比較研究。
- 表達系統(tǒng)全面：提供五大表達系統(tǒng)，包括大腸桿菌、酵母、桿狀病毒-昆蟲細(xì)胞、哺乳動物細(xì)胞以及特有的無細(xì)胞表達系統(tǒng)。該系統(tǒng)省去質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、破碎離心等步驟，顯著提升蛋白制備效率。
- 多標(biāo)簽選擇：支持His、GST、Flag、MBP等多種標(biāo)簽，便于后續(xù)純化、檢測與功能研究。
- 多領(lǐng)域應(yīng)用：適用于抗原制備、細(xì)胞與分析、蛋白互作、藥物研發(fā)、酶活性檢測、ELISA標(biāo)準(zhǔn)品、體內(nèi)外研究、質(zhì)譜與結(jié)構(gòu)分析等廣泛場景。
- 無動物源成分：我們已建立全程無動物副產(chǎn)品接觸的重組蛋白生產(chǎn)線，確保產(chǎn)品的高純凈性與一致性，適用于對來源敏感的各類實驗。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì)，或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的重組蛋白，請查看華美生物蛋白定制服務(wù)。
通過選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
| 產(chǎn)品名稱 | 貨號 | 規(guī)格 |
|---|---|---|
| Recombinant Yarrowia lipolytica UPF0507 protein YALI0E18612g (YALI0E18612g), partial | CSB-YP715411YAP CSB-EP715411YAP CSB-BP715411YAP CSB-MP715411YAP CSB-EP715411YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica Protein transport protein SEC23 (SEC23), partial | CSB-YP715412YAP CSB-EP715412YAP CSB-BP715412YAP CSB-MP715412YAP CSB-EP715412YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica 60S ribosomal protein L7 (RPL7) | CSB-YP715413YAP CSB-EP715413YAP CSB-BP715413YAP CSB-MP715413YAP CSB-EP715413YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica Mediator of RNA polymerase II transcription subunit 19 (ROX3) | CSB-YP715414YAP CSB-EP715414YAP CSB-BP715414YAP CSB-MP715414YAP CSB-EP715414YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica Inositol-pentakisphosphate 2-kinase (IPK1) | CSB-YP715415YAP CSB-EP715415YAP CSB-BP715415YAP CSB-MP715415YAP CSB-EP715415YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica F-actin-capping protein subunit alpha (CAP1) | CSB-YP715416YAP CSB-EP715416YAP CSB-BP715416YAP CSB-MP715416YAP CSB-EP715416YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica Antagonist of mitotic exit network protein 1 (AMN1), partial | CSB-YP715417YAP CSB-EP715417YAP CSB-BP715417YAP CSB-MP715417YAP CSB-EP715417YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica Homoaconitase, mitochondrial (LYS4), partial | CSB-YP715418YAP CSB-EP715418YAP CSB-BP715418YAP CSB-MP715418YAP CSB-EP715418YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica RNA polymerase II transcription factor B subunit 3 (TFB3) | CSB-YP715419YAP CSB-EP715419YAP CSB-BP715419YAP CSB-MP715419YAP CSB-EP715419YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica SWR1-complex protein 5 (SWC5) | CSB-YP715420YAP CSB-EP715420YAP CSB-BP715420YAP CSB-MP715420YAP CSB-EP715420YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica GPI ethanolamine phosphate transferase 2 (LAS21), partial | CSB-YP715421YAP CSB-EP715421YAP CSB-BP715421YAP CSB-MP715421YAP CSB-EP715421YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica Mitochondrial distribution and morphology protein 34 (MDM34) | CSB-YP715422YAP CSB-EP715422YAP CSB-BP715422YAP CSB-MP715422YAP CSB-EP715422YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica Acetylornithine aminotransferase, mitochondrial (ARG8), partial | CSB-YP715423YAP CSB-EP715423YAP CSB-BP715423YAP CSB-MP715423YAP CSB-EP715423YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica Mediator of RNA polymerase II transcription subunit 5 (NUT1), partial | CSB-YP715424YAP CSB-EP715424YAP CSB-BP715424YAP CSB-MP715424YAP CSB-EP715424YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica Chitin synthase export chaperone (CHS7), partial | CSB-YP715425YAP1 CSB-EP715425YAP1 CSB-BP715425YAP1 CSB-MP715425YAP1 CSB-EP715425YAP1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica Protein PNS1 (PNS1), partial | CSB-YP715426YAP CSB-EP715426YAP CSB-BP715426YAP CSB-MP715426YAP CSB-EP715426YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica Histone transcription regulator 3 homolog (HIR3), partial | CSB-YP715427YAP CSB-EP715427YAP CSB-BP715427YAP CSB-MP715427YAP CSB-EP715427YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica Chromatin modification-related protein EAF3 (EAF3) | CSB-YP715428YAP CSB-EP715428YAP CSB-BP715428YAP CSB-MP715428YAP CSB-EP715428YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica GDP-Man:Man (3)GlcNAc (2)-PP-Dol alpha-1,2-mannosyltransferase (ALG11), partial | CSB-YP715429YAP1 CSB-EP715429YAP1 CSB-BP715429YAP1 CSB-MP715429YAP1 CSB-EP715429YAP1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Yarrowia lipolytica DNA-directed RNA polymerases I, II, and III subunit RPABC1 (RPB5) | CSB-YP715430YAP CSB-EP715430YAP CSB-BP715430YAP CSB-MP715430YAP CSB-EP715430YAP-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。
第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條：如何選擇合適的表達載體?
表達載體是一種DNA分子，它攜帶特定的基因進入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。
根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022
