重組蛋白
重組蛋白是解析生物相互作用的關(guān)鍵工具，廣泛應(yīng)用于各類研究實(shí)驗(yàn)中。無(wú)論是商業(yè)化產(chǎn)品，還是為特定研究定制的蛋白，它們都在多種科學(xué)應(yīng)用中發(fā)揮著不可替代的作用。
若您需要優(yōu)質(zhì)重組蛋白——已驗(yàn)證的生物活性、低內(nèi)毒素水平、高純度——來(lái)為您的研究提供可靠支持，這里正是您的理想之選。
歷經(jīng)18年積累，華美生物已構(gòu)建覆蓋原核與真核的五大蛋白質(zhì)表達(dá)系統(tǒng)。我們擁有包括桿狀病毒外膜蛋白（OMP）表達(dá)技術(shù)、膜蛋白表達(dá)技術(shù)等在內(nèi)的多項(xiàng)蛋白表達(dá)與純化專有技術(shù)，致力于為您提供高質(zhì)量的重組蛋白解決方案。
產(chǎn)品特點(diǎn)
- 覆蓋廣泛：擁有超過(guò)16,000種重組蛋白，涵蓋人類、小鼠、大鼠、兔、豚鼠、雞、猴、豬等多種物種的基因與靶點(diǎn)，支持跨物種比較研究。
- 表達(dá)系統(tǒng)全面：提供五大表達(dá)系統(tǒng)，包括大腸桿菌、酵母、桿狀病毒-昆蟲(chóng)細(xì)胞、哺乳動(dòng)物細(xì)胞以及特有的無(wú)細(xì)胞表達(dá)系統(tǒng)。該系統(tǒng)省去質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、破碎離心等步驟，顯著提升蛋白制備效率。
- 多標(biāo)簽選擇：支持His、GST、Flag、MBP等多種標(biāo)簽，便于后續(xù)純化、檢測(cè)與功能研究。
- 多領(lǐng)域應(yīng)用：適用于抗原制備、細(xì)胞與分析、蛋白互作、藥物研發(fā)、酶活性檢測(cè)、ELISA標(biāo)準(zhǔn)品、體內(nèi)外研究、質(zhì)譜與結(jié)構(gòu)分析等廣泛場(chǎng)景。
- 無(wú)動(dòng)物源成分：我們已建立全程無(wú)動(dòng)物副產(chǎn)品接觸的重組蛋白生產(chǎn)線，確保產(chǎn)品的高純凈性與一致性，適用于對(duì)來(lái)源敏感的各類實(shí)驗(yàn)。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì)，或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的重組蛋白，請(qǐng)查看華美生物蛋白定制服務(wù)。
通過(guò)選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
| 產(chǎn)品名稱 | 貨號(hào) | 規(guī)格 |
|---|---|---|
| Recombinant Geobacillus kaustophilus 50S ribosomal protein L19 (rplS) | CSB-YP708548GAAA CSB-EP708548GAAA CSB-BP708548GAAA CSB-MP708548GAAA CSB-EP708548GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus UPF0122 protein GK1195 (GK1195) | CSB-YP708549GAAA CSB-EP708549GAAA CSB-BP708549GAAA CSB-MP708549GAAA CSB-EP708549GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Dihydroorotate dehydrogenase B (NAD (+)), catalytic subunit (pyrD) | CSB-YP708550GAAA CSB-EP708550GAAA CSB-BP708550GAAA CSB-MP708550GAAA CSB-EP708550GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase (murE) | CSB-YP708551GAAA CSB-EP708551GAAA CSB-BP708551GAAA CSB-MP708551GAAA CSB-EP708551GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Phosphopantetheine adenylyltransferase (coaD) | CSB-YP708552GAAA CSB-EP708552GAAA CSB-BP708552GAAA CSB-MP708552GAAA CSB-EP708552GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus UPF0356 protein GK1054 (GK1054) | CSB-YP708553GAAA CSB-EP708553GAAA CSB-BP708553GAAA CSB-MP708553GAAA CSB-EP708553GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase (mtnX) | CSB-YP708554GAAA CSB-EP708554GAAA CSB-BP708554GAAA CSB-MP708554GAAA CSB-EP708554GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Acetylglutamate kinase (argB) | CSB-YP708555GAAA CSB-EP708555GAAA CSB-BP708555GAAA CSB-MP708555GAAA CSB-EP708555GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus ATP-dependent helicase/nuclease subunit A (addA), partial | CSB-YP708556GAAA CSB-EP708556GAAA CSB-BP708556GAAA CSB-MP708556GAAA CSB-EP708556GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus HTH-type transcriptional regulator hpr (hpr) | CSB-YP708557GAAA CSB-EP708557GAAA CSB-BP708557GAAA CSB-MP708557GAAA CSB-EP708557GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Putative metal-dependent hydrolase GK0616 (GK0616) | CSB-YP708558GAAA CSB-EP708558GAAA CSB-BP708558GAAA CSB-MP708558GAAA CSB-EP708558GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus UPF0374 protein GK0468 (GK0468) | CSB-YP708559GAAA CSB-EP708559GAAA CSB-BP708559GAAA CSB-MP708559GAAA CSB-EP708559GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus UPF0753 protein GK0443 (GK0443), partial | CSB-YP708560GAAA CSB-EP708560GAAA CSB-BP708560GAAA CSB-MP708560GAAA CSB-EP708560GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Sulfate adenylyltransferase (sat) | CSB-YP708561GAAA CSB-EP708561GAAA CSB-BP708561GAAA CSB-MP708561GAAA CSB-EP708561GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus DNA ligase (ligA), partial | CSB-YP708562GAAA CSB-EP708562GAAA CSB-BP708562GAAA CSB-MP708562GAAA CSB-EP708562GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus GMP synthase [glutamine-hydrolyzing] (guaA), partial | CSB-YP708563GAAA CSB-EP708563GAAA CSB-BP708563GAAA CSB-MP708563GAAA CSB-EP708563GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus 1-pyrroline-5-carboxylate dehydrogenase (rocA), partial | CSB-YP708564GAAA CSB-EP708564GAAA CSB-BP708564GAAA CSB-MP708564GAAA CSB-EP708564GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Anti-sigma-W factor rsiW (rsiW), partial | CSB-YP708565GAAA CSB-EP708565GAAA CSB-BP708565GAAA CSB-MP708565GAAA CSB-EP708565GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Energy-coupling factor transporter ATP-binding protein EcfA 2 (ecfA2) | CSB-YP708566GAAA CSB-EP708566GAAA CSB-BP708566GAAA CSB-MP708566GAAA CSB-EP708566GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus 50S ribosomal protein L36 (rpmJ) | CSB-YP708567GAAA CSB-EP708567GAAA CSB-BP708567GAAA CSB-MP708567GAAA CSB-EP708567GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。
第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條：如何選擇合適的表達(dá)載體?
表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過(guò)多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。
根據(jù)重組蛋白的不同應(yīng)用，對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn)，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022
