重組蛋白
重組蛋白是解析生物相互作用的關(guān)鍵工具，廣泛應(yīng)用于各類研究實驗中。無論是商業(yè)化產(chǎn)品，還是為特定研究定制的蛋白，它們都在多種科學(xué)應(yīng)用中發(fā)揮著不可替代的作用。
若您需要優(yōu)質(zhì)重組蛋白——已驗證的生物活性、低內(nèi)毒素水平、高純度——來為您的研究提供可靠支持，這里正是您的理想之選。
歷經(jīng)18年積累，華美生物已構(gòu)建覆蓋原核與真核的五大蛋白質(zhì)表達(dá)系統(tǒng)。我們擁有包括桿狀病毒外膜蛋白（OMP）表達(dá)技術(shù)、膜蛋白表達(dá)技術(shù)等在內(nèi)的多項蛋白表達(dá)與純化專有技術(shù)，致力于為您提供高質(zhì)量的重組蛋白解決方案。
產(chǎn)品特點
- 覆蓋廣泛：擁有超過16,000種重組蛋白，涵蓋人類、小鼠、大鼠、兔、豚鼠、雞、猴、豬等多種物種的基因與靶點，支持跨物種比較研究。
- 表達(dá)系統(tǒng)全面：提供五大表達(dá)系統(tǒng)，包括大腸桿菌、酵母、桿狀病毒-昆蟲細(xì)胞、哺乳動物細(xì)胞以及特有的無細(xì)胞表達(dá)系統(tǒng)。該系統(tǒng)省去質(zhì)粒轉(zhuǎn)化、細(xì)胞培養(yǎng)、破碎離心等步驟，顯著提升蛋白制備效率。
- 多標(biāo)簽選擇：支持His、GST、Flag、MBP等多種標(biāo)簽，便于后續(xù)純化、檢測與功能研究。
- 多領(lǐng)域應(yīng)用：適用于抗原制備、細(xì)胞與分析、蛋白互作、藥物研發(fā)、酶活性檢測、ELISA標(biāo)準(zhǔn)品、體內(nèi)外研究、質(zhì)譜與結(jié)構(gòu)分析等廣泛場景。
- 無動物源成分：我們已建立全程無動物副產(chǎn)品接觸的重組蛋白生產(chǎn)線，確保產(chǎn)品的高純凈性與一致性，適用于對來源敏感的各類實驗。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì)，或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的重組蛋白，請查看華美生物蛋白定制服務(wù)。
通過選擇目標(biāo)名稱的首字母瀏覽產(chǎn)品。
| 產(chǎn)品名稱 | 貨號 | 規(guī)格 |
|---|---|---|
| Recombinant Geobacillus kaustophilus (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB), partial | CSB-YP689399GAAA CSB-EP689399GAAA CSB-BP689399GAAA CSB-MP689399GAAA CSB-EP689399GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Zinc metalloprotease rasP (rasP), partial | CSB-YP689400GAAA1 CSB-EP689400GAAA1 CSB-BP689400GAAA1 CSB-MP689400GAAA1 CSB-EP689400GAAA1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Acyl carrier protein (acpP) | CSB-YP689401GAAA CSB-EP689401GAAA CSB-BP689401GAAA CSB-MP689401GAAA CSB-EP689401GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Methionyl-tRNA formyltransferase (fmt) | CSB-YP689402GAAA CSB-EP689402GAAA CSB-BP689402GAAA CSB-MP689402GAAA CSB-EP689402GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Orotidine 5'-phosphate decarboxylase (pyrF) | CSB-YP689403GAAA CSB-EP689403GAAA CSB-BP689403GAAA CSB-MP689403GAAA CSB-EP689403GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Lipoprotein signal peptidase (lspA), partial | CSB-YP689404GAAA1 CSB-EP689404GAAA1 CSB-BP689404GAAA1 CSB-MP689404GAAA1 CSB-EP689404GAAA1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus UPF0298 protein GK1096 (GK1096) | CSB-YP689405GAAA CSB-EP689405GAAA CSB-BP689405GAAA CSB-MP689405GAAA CSB-EP689405GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus UPF0180 protein GK1051 (GK1051) | CSB-YP689406GAAA CSB-EP689406GAAA CSB-BP689406GAAA CSB-MP689406GAAA CSB-EP689406GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus 2,3-diketo-5-methylthiopentyl-1-phosphate enolase (mtnW) | CSB-YP689407GAAA CSB-EP689407GAAA CSB-BP689407GAAA CSB-MP689407GAAA CSB-EP689407GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Probable inorganic polyphosphate/ATP-NAD kinase 1 (ppnK1) | CSB-YP689408GAAA CSB-EP689408GAAA CSB-BP689408GAAA CSB-MP689408GAAA CSB-EP689408GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus N-acetyl-gamma-glutamyl-phosphate reductase (argC) | CSB-YP689409GAAA CSB-EP689409GAAA CSB-BP689409GAAA CSB-MP689409GAAA CSB-EP689409GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus UPF0344 protein GK0697 (GK0697), partial | CSB-YP689410GAAA1 CSB-EP689410GAAA1 CSB-BP689410GAAA1 CSB-MP689410GAAA1 CSB-EP689410GAAA1-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Foldase protein prsA (prsA) | CSB-YP689411GAAA CSB-EP689411GAAA CSB-BP689411GAAA CSB-MP689411GAAA CSB-EP689411GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Thiazole synthase (thiG) | CSB-YP689412GAAA CSB-EP689412GAAA CSB-BP689412GAAA CSB-MP689412GAAA CSB-EP689412GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Glutamate-1-semialdehyde 2,1-aminomutase 1 (hemL1) | CSB-YP689413GAAA CSB-EP689413GAAA CSB-BP689413GAAA CSB-MP689413GAAA CSB-EP689413GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus UPF0435 protein GK0418 (GK0418) | CSB-YP689414GAAA CSB-EP689414GAAA CSB-BP689414GAAA CSB-MP689414GAAA CSB-EP689414GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Lactate utilization protein B (lutB) | CSB-YP689415GAAA CSB-EP689415GAAA CSB-BP689415GAAA CSB-MP689415GAAA CSB-EP689415GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B (gatB) | CSB-YP689416GAAA CSB-EP689416GAAA CSB-BP689416GAAA CSB-MP689416GAAA CSB-EP689416GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Phosphoribosylformylglycinamidine cyclo-ligase (purM) | CSB-YP689417GAAA CSB-EP689417GAAA CSB-BP689417GAAA CSB-MP689417GAAA CSB-EP689417GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Geobacillus kaustophilus Undecaprenyl-diphosphatase (uppP), partial | CSB-YP689418GAAA CSB-EP689418GAAA CSB-BP689418GAAA CSB-MP689418GAAA CSB-EP689418GAAA-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。
第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條：如何選擇合適的表達(dá)載體?
表達(dá)載體是一種DNA分子，它攜帶特定的基因進入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。
根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022
