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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Geobacter metallireducens NADH-quinone oxidoreductase subunit A 2 (nuoA2), partial CSB-YP658658GBJ1
CSB-EP658658GBJ1
CSB-BP658658GBJ1
CSB-MP658658GBJ1
CSB-EP658658GBJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens tRNA 2-thiocytidine biosynthesis protein TtcA (ttcA) CSB-YP658659GBJ
CSB-EP658659GBJ
CSB-BP658659GBJ
CSB-MP658659GBJ
CSB-EP658659GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens UPF0234 protein Gmet_3206 (Gmet_3206) CSB-YP658660GBJ
CSB-EP658660GBJ
CSB-BP658660GBJ
CSB-MP658660GBJ
CSB-EP658660GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens GTPase obg (obg) CSB-YP658661GBJ
CSB-EP658661GBJ
CSB-BP658661GBJ
CSB-MP658661GBJ
CSB-EP658661GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens 7-cyano-7-deazaguanine synthase (queC) CSB-YP658662GBJ
CSB-EP658662GBJ
CSB-BP658662GBJ
CSB-MP658662GBJ
CSB-EP658662GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens Glycine--tRNA ligase alpha subunit (glyQ) CSB-YP658663GBJ
CSB-EP658663GBJ
CSB-BP658663GBJ
CSB-MP658663GBJ
CSB-EP658663GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens 30S ribosomal protein S16 (rpsP) CSB-YP658664GBJ
CSB-EP658664GBJ
CSB-BP658664GBJ
CSB-MP658664GBJ
CSB-EP658664GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE) CSB-YP658665GBJ
CSB-EP658665GBJ
CSB-BP658665GBJ
CSB-MP658665GBJ
CSB-EP658665GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens Homoserine O-acetyltransferase (metX) CSB-YP658666GBJ
CSB-EP658666GBJ
CSB-BP658666GBJ
CSB-MP658666GBJ
CSB-EP658666GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP658667GBJ
CSB-EP658667GBJ
CSB-BP658667GBJ
CSB-MP658667GBJ
CSB-EP658667GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens N- (5'-phosphoribosyl)anthranilate isomerase (trpF) CSB-YP658668GBJ
CSB-EP658668GBJ
CSB-BP658668GBJ
CSB-MP658668GBJ
CSB-EP658668GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens Probable rRNA maturation factor (Gmet_2370) CSB-YP658669GBJ
CSB-EP658669GBJ
CSB-BP658669GBJ
CSB-MP658669GBJ
CSB-EP658669GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP658670GBJ
CSB-EP658670GBJ
CSB-BP658670GBJ
CSB-MP658670GBJ
CSB-EP658670GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP658671GBJ
CSB-EP658671GBJ
CSB-BP658671GBJ
CSB-MP658671GBJ
CSB-EP658671GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens Histidine--tRNA ligase (hisS) CSB-YP658672GBJ
CSB-EP658672GBJ
CSB-BP658672GBJ
CSB-MP658672GBJ
CSB-EP658672GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens ATP-dependent Clp protease ATP-binding subunit ClpX (clpX) CSB-YP658673GBJ
CSB-EP658673GBJ
CSB-BP658673GBJ
CSB-MP658673GBJ
CSB-EP658673GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens Elongation factor 4 (lepA), partial CSB-YP658674GBJ
CSB-EP658674GBJ
CSB-BP658674GBJ
CSB-MP658674GBJ
CSB-EP658674GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens Pantothenate synthetase (panC) CSB-YP658675GBJ
CSB-EP658675GBJ
CSB-BP658675GBJ
CSB-MP658675GBJ
CSB-EP658675GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens 50S ribosomal protein L32 (rpmF) CSB-YP658676GBJ
CSB-EP658676GBJ
CSB-BP658676GBJ
CSB-MP658676GBJ
CSB-EP658676GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter metallireducens Translation initiation factor IF-2 (infB), partial CSB-YP658677GBJ
CSB-EP658677GBJ
CSB-BP658677GBJ
CSB-MP658677GBJ
CSB-EP658677GBJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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