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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shigella dysenteriae serotype 1 UPF0115 protein yfcN (yfcN) CSB-YP657598SGF
CSB-EP657598SGF
CSB-BP657598SGF
CSB-MP657598SGF
CSB-EP657598SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 tRNA pseudouridine synthase A (truA) CSB-YP657599SGF
CSB-EP657599SGF
CSB-BP657599SGF
CSB-MP657599SGF
CSB-EP657599SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 NADH-quinone oxidoreductase subunit I (nuoI) CSB-YP657600SGF
CSB-EP657600SGF
CSB-BP657600SGF
CSB-MP657600SGF
CSB-EP657600SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD (arnD) CSB-YP657601SGF
CSB-EP657601SGF
CSB-BP657601SGF
CSB-MP657601SGF
CSB-EP657601SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Hydroxylamine reductase (hcp), partial CSB-YP657602SGF
CSB-EP657602SGF
CSB-BP657602SGF
CSB-MP657602SGF
CSB-EP657602SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Cytidylate kinase (cmk) CSB-YP657603SGF
CSB-EP657603SGF
CSB-BP657603SGF
CSB-MP657603SGF
CSB-EP657603SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Chromosome partition protein MukB (mukB), partial CSB-YP657604SGF
CSB-EP657604SGF
CSB-BP657604SGF
CSB-MP657604SGF
CSB-EP657604SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Nickel/cobalt efflux system rcnA (rcnA), partial CSB-YP657605SGF1
CSB-EP657605SGF1
CSB-BP657605SGF1
CSB-MP657605SGF1
CSB-EP657605SGF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 ATP phosphoribosyltransferase (hisG) CSB-YP657606SGF
CSB-EP657606SGF
CSB-BP657606SGF
CSB-MP657606SGF
CSB-EP657606SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 S-formylglutathione hydrolase yeiG (yeiG) CSB-YP657607SGF
CSB-EP657607SGF
CSB-BP657607SGF
CSB-MP657607SGF
CSB-EP657607SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Flagellar P-ring protein (flgI) CSB-YP657608SGF
CSB-EP657608SGF
CSB-BP657608SGF
CSB-MP657608SGF
CSB-EP657608SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Beta-hexosaminidase (nagZ) CSB-YP657609SGF
CSB-EP657609SGF
CSB-BP657609SGF
CSB-MP657609SGF
CSB-EP657609SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 tRNA-specific 2-thiouridylase mnmA (mnmA) CSB-YP657610SGF
CSB-EP657610SGF
CSB-BP657610SGF
CSB-MP657610SGF
CSB-EP657610SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 UPF0266 membrane protein yobD (yobD), partial CSB-YP657611SGF1
CSB-EP657611SGF1
CSB-BP657611SGF1
CSB-MP657611SGF1
CSB-EP657611SGF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Tyrosine--tRNA ligase (tyrS) CSB-YP657612SGF
CSB-EP657612SGF
CSB-BP657612SGF
CSB-MP657612SGF
CSB-EP657612SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 OriC-binding nucleoid-associated protein (cnu) CSB-YP657613SGF
CSB-EP657613SGF
CSB-BP657613SGF
CSB-MP657613SGF
CSB-EP657613SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Integration host factor subunit alpha (ihfA) CSB-YP657614SGF
CSB-EP657614SGF
CSB-BP657614SGF
CSB-MP657614SGF
CSB-EP657614SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 UPF0181 protein yoaH (yoaH) CSB-YP657615SGF
CSB-EP657615SGF
CSB-BP657615SGF
CSB-MP657615SGF
CSB-EP657615SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Trans-aconitate 2-methyltransferase (tam) CSB-YP657616SGF
CSB-EP657616SGF
CSB-BP657616SGF
CSB-MP657616SGF
CSB-EP657616SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella dysenteriae serotype 1 Putative protein ves (ves) CSB-YP657617SGF
CSB-EP657617SGF
CSB-BP657617SGF
CSB-MP657617SGF
CSB-EP657617SGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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