重組蛋白
重組蛋白是解析生物相互作用的關(guān)鍵工具，廣泛應(yīng)用于各類研究實驗中。無論是商業(yè)化產(chǎn)品，還是為特定研究定制的蛋白，它們都在多種科學(xué)應(yīng)用中發(fā)揮著不可替代的作用。
若您需要優(yōu)質(zhì)重組蛋白——已驗證的生物活性、低內(nèi)毒素水平、高純度——來為您的研究提供可靠支持，這里正是您的理想之選。
歷經(jīng)18年積累，華美生物已構(gòu)建覆蓋原核與真核的五大蛋白質(zhì)表達系統(tǒng)。我們擁有包括桿狀病毒外膜蛋白（OMP）表達技術(shù)、膜蛋白表達技術(shù)等在內(nèi)的多項蛋白表達與純化專有技術(shù)，致力于為您提供高質(zhì)量的重組蛋白解決方案。
產(chǎn)品特點
- 覆蓋廣泛：擁有超過16,000種重組蛋白，涵蓋人類、小鼠、大鼠、兔、豚鼠、雞、猴、豬等多種物種的基因與靶點，支持跨物種比較研究。
- 表達系統(tǒng)全面：提供五大表達系統(tǒng)，包括大腸桿菌、酵母、桿狀病毒-昆蟲細胞、哺乳動物細胞以及特有的無細胞表達系統(tǒng)。該系統(tǒng)省去質(zhì)粒轉(zhuǎn)化、細胞培養(yǎng)、破碎離心等步驟，顯著提升蛋白制備效率。
- 多標簽選擇：支持His、GST、Flag、MBP等多種標簽，便于后續(xù)純化、檢測與功能研究。
- 多領(lǐng)域應(yīng)用：適用于抗原制備、細胞與分析、蛋白互作、藥物研發(fā)、酶活性檢測、ELISA標準品、體內(nèi)外研究、質(zhì)譜與結(jié)構(gòu)分析等廣泛場景。
- 無動物源成分：我們已建立全程無動物副產(chǎn)品接觸的重組蛋白生產(chǎn)線，確保產(chǎn)品的高純凈性與一致性，適用于對來源敏感的各類實驗。
重組蛋白目錄
以下是所有重組蛋白的目錄。您可以在目錄中找到適合您研究的蛋白質(zhì)，或在本網(wǎng)站頭部的搜索框中搜索它們。
如果您找不到您需要的重組蛋白，請查看華美生物蛋白定制服務(wù)。
通過選擇目標名稱的首字母瀏覽產(chǎn)品。
| 產(chǎn)品名稱 | 貨號 | 規(guī)格 |
|---|---|---|
| Recombinant Methanosphaera stadtmanae DNA polymerase II large subunit (polC), partial | CSB-YP647523MAAR CSB-EP647523MAAR CSB-BP647523MAAR CSB-MP647523MAAR CSB-EP647523MAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanosphaera stadtmanae Riboflavin kinase (ribK) | CSB-YP647524MAAR CSB-EP647524MAAR CSB-BP647524MAAR CSB-MP647524MAAR CSB-EP647524MAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanosphaera stadtmanae Dihydrodipicolinate synthase (dapA) | CSB-YP647525MAAR CSB-EP647525MAAR CSB-BP647525MAAR CSB-MP647525MAAR CSB-EP647525MAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanosphaera stadtmanae Probable DNA primase small subunit (priA) | CSB-YP647526MAAR CSB-EP647526MAAR CSB-BP647526MAAR CSB-MP647526MAAR CSB-EP647526MAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanosphaera stadtmanae Imidazole glycerol phosphate synthase subunit HisF (hisF) | CSB-YP647527MAAR CSB-EP647527MAAR CSB-BP647527MAAR CSB-MP647527MAAR CSB-EP647527MAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Methanosphaera stadtmanae Cell division control protein 6 homolog (cdc6) | CSB-YP647528MAAR CSB-EP647528MAAR CSB-BP647528MAAR CSB-MP647528MAAR CSB-EP647528MAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Aster yellows witches'-broom phytoplasma Ribosomal RNA small subunit methyltransferase H (rsmH) | CSB-YP647529AAAR CSB-EP647529AAAR CSB-BP647529AAAR CSB-MP647529AAAR CSB-EP647529AAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Aster yellows witches'-broom phytoplasma GTPase obg (obg) | CSB-YP647530AAAR CSB-EP647530AAAR CSB-BP647530AAAR CSB-MP647530AAAR CSB-EP647530AAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Aster yellows witches'-broom phytoplasma 30S ribosomal protein S15 (rpsO) | CSB-YP647531AAAR CSB-EP647531AAAR CSB-BP647531AAAR CSB-MP647531AAAR CSB-EP647531AAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Aster yellows witches'-broom phytoplasma Uridylate kinase (pyrH) | CSB-YP647532AAAR CSB-EP647532AAAR CSB-BP647532AAAR CSB-MP647532AAAR CSB-EP647532AAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Aster yellows witches'-broom phytoplasma 30S ribosomal protein S10 (rpsJ) | CSB-YP647533AAAR CSB-EP647533AAAR CSB-BP647533AAAR CSB-MP647533AAAR CSB-EP647533AAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Aster yellows witches'-broom phytoplasma 50S ribosomal protein L14 (rplN) | CSB-YP647534AAAR CSB-EP647534AAAR CSB-BP647534AAAR CSB-MP647534AAAR CSB-EP647534AAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Aster yellows witches'-broom phytoplasma 30S ribosomal protein S5 (rpsE) | CSB-YP647535AAAR CSB-EP647535AAAR CSB-BP647535AAAR CSB-MP647535AAAR CSB-EP647535AAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Aster yellows witches'-broom phytoplasma tRNA pseudouridine synthase A (truA) | CSB-YP647536AAAR CSB-EP647536AAAR CSB-BP647536AAAR CSB-MP647536AAAR CSB-EP647536AAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Aster yellows witches'-broom phytoplasma Polyribonucleotide nucleotidyltransferase (pnp), partial | CSB-YP647537AAAR CSB-EP647537AAAR CSB-BP647537AAAR CSB-MP647537AAAR CSB-EP647537AAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Aster yellows witches'-broom phytoplasma Elongation factor Tu (tuf) | CSB-YP647538AAAR CSB-EP647538AAAR CSB-BP647538AAAR CSB-MP647538AAAR CSB-EP647538AAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Aster yellows witches'-broom phytoplasma Thymidylate synthase (thyA) | CSB-YP647539AAAR CSB-EP647539AAAR CSB-BP647539AAAR CSB-MP647539AAAR CSB-EP647539AAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Aster yellows witches'-broom phytoplasma 30S ribosomal protein S4 (rpsD) | CSB-YP647541AAAR CSB-EP647541AAAR CSB-BP647541AAAR CSB-MP647541AAAR CSB-EP647541AAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Aster yellows witches'-broom phytoplasma 30S ribosomal protein S21 (rpsU) | CSB-YP647542AAAR CSB-EP647542AAAR CSB-BP647542AAAR CSB-MP647542AAAR CSB-EP647542AAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
| Recombinant Aster yellows witches'-broom phytoplasma 30S ribosomal protein S18 (rpsR) | CSB-YP647543AAAR CSB-EP647543AAAR CSB-BP647543AAAR CSB-MP647543AAAR CSB-EP647543AAAR-B |
20μg/100μg/1mg(1mg*1 or 500ug*2) |
有用資源
什么是重組蛋白?
重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示，可以幫助你做出明智的決策。
第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。
第二條：如何選擇合適的表達載體?
表達載體是一種DNA分子，它攜帶特定的基因進入宿主細胞，并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達，經(jīng)過多年的發(fā)展，表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示，本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。
根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達服務(wù)，您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等，您可以點擊橙色按鈕為我們在線留言。
引用資料
Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023
Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023
Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023
Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023
Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022
