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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Rhizobium etli Cell division topological specificity factor (minE) CSB-YP642462RKK
CSB-EP642462RKK
CSB-BP642462RKK
CSB-MP642462RKK
CSB-EP642462RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Ribose import ATP-binding protein RbsA 2 (rbsA2), partial CSB-YP642463RKK
CSB-EP642463RKK
CSB-BP642463RKK
CSB-MP642463RKK
CSB-EP642463RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Acetyl-coenzyme A synthetase (acsA), partial CSB-YP642464RKK
CSB-EP642464RKK
CSB-BP642464RKK
CSB-MP642464RKK
CSB-EP642464RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP642465RKK
CSB-EP642465RKK
CSB-BP642465RKK
CSB-MP642465RKK
CSB-EP642465RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli 50S ribosomal protein L32 (rpmF) CSB-YP642466RKK
CSB-EP642466RKK
CSB-BP642466RKK
CSB-MP642466RKK
CSB-EP642466RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP642467RKK
CSB-EP642467RKK
CSB-BP642467RKK
CSB-MP642467RKK
CSB-EP642467RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Argininosuccinate synthase (argG) CSB-YP642468RKK
CSB-EP642468RKK
CSB-BP642468RKK
CSB-MP642468RKK
CSB-EP642468RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli ATP synthase epsilon chain (atpC) CSB-YP642469RKK
CSB-EP642469RKK
CSB-BP642469RKK
CSB-MP642469RKK
CSB-EP642469RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli 7-cyano-7-deazaguanine synthase (queC) CSB-YP642470RKK
CSB-EP642470RKK
CSB-BP642470RKK
CSB-MP642470RKK
CSB-EP642470RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli UPF0176 protein RHE_CH03638 (RHE_CH03638) CSB-YP642471RKK
CSB-EP642471RKK
CSB-BP642471RKK
CSB-MP642471RKK
CSB-EP642471RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli TPR repeat-containing protein RHE_CH03534.1 (RHE_CH03534.1) CSB-YP642472RKK
CSB-EP642472RKK
CSB-BP642472RKK
CSB-MP642472RKK
CSB-EP642472RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Protein TolB (tolB) CSB-YP642473RKK
CSB-EP642473RKK
CSB-BP642473RKK
CSB-MP642473RKK
CSB-EP642473RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Urease subunit gamma (ureA) CSB-YP642474RKK
CSB-EP642474RKK
CSB-BP642474RKK
CSB-MP642474RKK
CSB-EP642474RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli GTPase Der (der) CSB-YP642475RKK
CSB-EP642475RKK
CSB-BP642475RKK
CSB-MP642475RKK
CSB-EP642475RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Peptidyl-tRNA hydrolase (pth) CSB-YP642476RKK
CSB-EP642476RKK
CSB-BP642476RKK
CSB-MP642476RKK
CSB-EP642476RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP642477RKK
CSB-EP642477RKK
CSB-BP642477RKK
CSB-MP642477RKK
CSB-EP642477RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli tRNA dimethylallyltransferase (miaA) CSB-YP642478RKK
CSB-EP642478RKK
CSB-BP642478RKK
CSB-MP642478RKK
CSB-EP642478RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase (cobT) CSB-YP642479RKK
CSB-EP642479RKK
CSB-BP642479RKK
CSB-MP642479RKK
CSB-EP642479RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP642480RKK
CSB-EP642480RKK
CSB-BP642480RKK
CSB-MP642480RKK
CSB-EP642480RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium etli Putative membrane protein insertion efficiency factor (RHE_CH02199) CSB-YP642481RKK
CSB-EP642481RKK
CSB-BP642481RKK
CSB-MP642481RKK
CSB-EP642481RKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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