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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Lawsonia intracellularis 50S ribosomal protein L24 (rplX) CSB-YP638130LAAL
CSB-EP638130LAAL
CSB-BP638130LAAL
CSB-MP638130LAAL
CSB-EP638130LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 50S ribosomal protein L23 (rplW) CSB-YP638131LAAL
CSB-EP638131LAAL
CSB-BP638131LAAL
CSB-MP638131LAAL
CSB-EP638131LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Elongation factor Tu (tuf) CSB-YP638132LAAL
CSB-EP638132LAAL
CSB-BP638132LAAL
CSB-MP638132LAAL
CSB-EP638132LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Cytidylate kinase (cmk) CSB-YP638133LAAL
CSB-EP638133LAAL
CSB-BP638133LAAL
CSB-MP638133LAAL
CSB-EP638133LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Sugar fermentation stimulation protein homolog (sfsA) CSB-YP638134LAAL
CSB-EP638134LAAL
CSB-BP638134LAAL
CSB-MP638134LAAL
CSB-EP638134LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB (miaB) CSB-YP638135LAAL
CSB-EP638135LAAL
CSB-BP638135LAAL
CSB-MP638135LAAL
CSB-EP638135LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Trigger factor (tig) CSB-YP638136LAAL
CSB-EP638136LAAL
CSB-BP638136LAAL
CSB-MP638136LAAL
CSB-EP638136LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 5'-nucleotidase surE (surE) CSB-YP638137LAAL
CSB-EP638137LAAL
CSB-BP638137LAAL
CSB-MP638137LAAL
CSB-EP638137LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis D-alanine--D-alanine ligase (ddl) CSB-YP638138LAAL
CSB-EP638138LAAL
CSB-BP638138LAAL
CSB-MP638138LAAL
CSB-EP638138LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 10 kDa chaperonin (groS) CSB-YP638139LAAL
CSB-EP638139LAAL
CSB-BP638139LAAL
CSB-MP638139LAAL
CSB-EP638139LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 50S ribosomal protein L13 (rplM) CSB-YP638140LAAL
CSB-EP638140LAAL
CSB-BP638140LAAL
CSB-MP638140LAAL
CSB-EP638140LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis UPF0042 nucleotide-binding protein LI0459 (LI0459) CSB-YP638141LAAL
CSB-EP638141LAAL
CSB-BP638141LAAL
CSB-MP638141LAAL
CSB-EP638141LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 1-deoxy-D-xylulose-5-phosphate synthase (dxs), partial CSB-YP638142LAAL
CSB-EP638142LAAL
CSB-BP638142LAAL
CSB-MP638142LAAL
CSB-EP638142LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Uridylate kinase (pyrH) CSB-YP638143LAAL
CSB-EP638143LAAL
CSB-BP638143LAAL
CSB-MP638143LAAL
CSB-EP638143LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Acetylglutamate kinase (argB) CSB-YP638144LAAL
CSB-EP638144LAAL
CSB-BP638144LAAL
CSB-MP638144LAAL
CSB-EP638144LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Glycine--tRNA ligase beta subunit (glyS), partial CSB-YP638145LAAL
CSB-EP638145LAAL
CSB-BP638145LAAL
CSB-MP638145LAAL
CSB-EP638145LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Probable hydrogenase nickel incorporation protein HypA (hypA) CSB-YP638146LAAL
CSB-EP638146LAAL
CSB-BP638146LAAL
CSB-MP638146LAAL
CSB-EP638146LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis 50S ribosomal protein L19 (rplS) CSB-YP638147LAAL
CSB-EP638147LAAL
CSB-BP638147LAAL
CSB-MP638147LAAL
CSB-EP638147LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP638148LAAL1
CSB-EP638148LAAL1
CSB-BP638148LAAL1
CSB-MP638148LAAL1
CSB-EP638148LAAL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lawsonia intracellularis Probable GTP-binding protein EngB (engB) CSB-YP638149LAAL
CSB-EP638149LAAL
CSB-BP638149LAAL
CSB-MP638149LAAL
CSB-EP638149LAAL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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