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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ralstonia metallidurans 50S ribosomal protein L36 (rpmJ) CSB-YP629732RJG
CSB-EP629732RJG
CSB-BP629732RJG
CSB-MP629732RJG
CSB-EP629732RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans 3-dehydroquinate synthase (aroB) CSB-YP629733RJG
CSB-EP629733RJG
CSB-BP629733RJG
CSB-MP629733RJG
CSB-EP629733RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Sec-independent protein translocase protein TatA (tatA), partial CSB-YP629734RJG
CSB-EP629734RJG
CSB-BP629734RJG
CSB-MP629734RJG
CSB-EP629734RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP629735RJG
CSB-EP629735RJG
CSB-BP629735RJG
CSB-MP629735RJG
CSB-EP629735RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans UPF0289 protein Rmet_3112 (Rmet_3112) CSB-YP629736RJG
CSB-EP629736RJG
CSB-BP629736RJG
CSB-MP629736RJG
CSB-EP629736RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Probable chorismate--pyruvate lyase (ubiC) CSB-YP629737RJG
CSB-EP629737RJG
CSB-BP629737RJG
CSB-MP629737RJG
CSB-EP629737RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Queuine tRNA-ribosyltransferase (tgt) CSB-YP629738RJG
CSB-EP629738RJG
CSB-BP629738RJG
CSB-MP629738RJG
CSB-EP629738RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Probable cytosol aminopeptidase (pepA), partial CSB-YP629739RJG
CSB-EP629739RJG
CSB-BP629739RJG
CSB-MP629739RJG
CSB-EP629739RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Aspartate carbamoyltransferase (pyrB) CSB-YP629740RJG
CSB-EP629740RJG
CSB-BP629740RJG
CSB-MP629740RJG
CSB-EP629740RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans 7-cyano-7-deazaguanine synthase (queC) CSB-YP629741RJG
CSB-EP629741RJG
CSB-BP629741RJG
CSB-MP629741RJG
CSB-EP629741RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Glutamyl-Q tRNA (Asp) synthetase (gluQ) CSB-YP629742RJG
CSB-EP629742RJG
CSB-BP629742RJG
CSB-MP629742RJG
CSB-EP629742RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans 30S ribosomal protein S21 1 (rpsU1) CSB-YP629743RJG
CSB-EP629743RJG
CSB-BP629743RJG
CSB-MP629743RJG
CSB-EP629743RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Ribonuclease 3 (rnc) CSB-YP629744RJG
CSB-EP629744RJG
CSB-BP629744RJG
CSB-MP629744RJG
CSB-EP629744RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Ribonuclease H (rnhA) CSB-YP629745RJG
CSB-EP629745RJG
CSB-BP629745RJG
CSB-MP629745RJG
CSB-EP629745RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans 50S ribosomal protein L31 type B (rpmE2) CSB-YP629746RJG
CSB-EP629746RJG
CSB-BP629746RJG
CSB-MP629746RJG
CSB-EP629746RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans ATP phosphoribosyltransferase regulatory subunit (hisZ) CSB-YP629747RJG
CSB-EP629747RJG
CSB-BP629747RJG
CSB-MP629747RJG
CSB-EP629747RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans tRNA pseudouridine synthase B (truB) CSB-YP629748RJG
CSB-EP629748RJG
CSB-BP629748RJG
CSB-MP629748RJG
CSB-EP629748RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC), partial CSB-YP629749RJG
CSB-EP629749RJG
CSB-BP629749RJG
CSB-MP629749RJG
CSB-EP629749RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Phosphoribosylglycinamide formyltransferase 2 (purT) CSB-YP629750RJG
CSB-EP629750RJG
CSB-BP629750RJG
CSB-MP629750RJG
CSB-EP629750RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia metallidurans Lipid-A-disaccharide synthase (lpxB) CSB-YP629751RJG
CSB-EP629751RJG
CSB-BP629751RJG
CSB-MP629751RJG
CSB-EP629751RJG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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