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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia pestis bv. Antiqua Proline--tRNA ligase (proS), partial CSB-YP627612YAG
CSB-EP627612YAG
CSB-BP627612YAG
CSB-MP627612YAG
CSB-EP627612YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Protein RnfH (rnfH) CSB-YP627613YAG
CSB-EP627613YAG
CSB-BP627613YAG
CSB-MP627613YAG
CSB-EP627613YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Phosphonates import ATP-binding protein PhnC (phnC) CSB-YP627614YAG
CSB-EP627614YAG
CSB-BP627614YAG
CSB-MP627614YAG
CSB-EP627614YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 23S rRNA (uracil (747)-C (5))-methyltransferase RlmC (rlmC) CSB-YP627615YAG
CSB-EP627615YAG
CSB-BP627615YAG
CSB-MP627615YAG
CSB-EP627615YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Integration host factor subunit beta (ihfB) CSB-YP627616YAG
CSB-EP627616YAG
CSB-BP627616YAG
CSB-MP627616YAG
CSB-EP627616YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Asparagine--tRNA ligase (asnS) CSB-YP627617YAG
CSB-EP627617YAG
CSB-BP627617YAG
CSB-MP627617YAG
CSB-EP627617YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Heat shock protein HspQ (hspQ) CSB-YP627618YAG
CSB-EP627618YAG
CSB-BP627618YAG
CSB-MP627618YAG
CSB-EP627618YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Uridine kinase (udk) CSB-YP627619YAG
CSB-EP627619YAG
CSB-BP627619YAG
CSB-MP627619YAG
CSB-EP627619YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua ATP phosphoribosyltransferase (hisG) CSB-YP627620YAG
CSB-EP627620YAG
CSB-BP627620YAG
CSB-MP627620YAG
CSB-EP627620YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0181 protein YPN_2349 (YPN_2349) CSB-YP627621YAG
CSB-EP627621YAG
CSB-BP627621YAG
CSB-MP627621YAG
CSB-EP627621YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Flavoprotein WrbA (wrbA) CSB-YP627622YAG
CSB-EP627622YAG
CSB-BP627622YAG
CSB-MP627622YAG
CSB-EP627622YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Fatty acid oxidation complex subunit alpha (fadJ), partial CSB-YP627623YAG
CSB-EP627623YAG
CSB-BP627623YAG
CSB-MP627623YAG
CSB-EP627623YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua NADH-quinone oxidoreductase subunit B (nuoB) CSB-YP627624YAG
CSB-EP627624YAG
CSB-BP627624YAG
CSB-MP627624YAG
CSB-EP627624YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0303 protein YPN_2129 (YPN_2129) CSB-YP627625YAG
CSB-EP627625YAG
CSB-BP627625YAG
CSB-MP627625YAG
CSB-EP627625YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Dihydroorotase (pyrC) CSB-YP627626YAG
CSB-EP627626YAG
CSB-BP627626YAG
CSB-MP627626YAG
CSB-EP627626YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Acyl carrier protein (acpP) CSB-YP627627YAG
CSB-EP627627YAG
CSB-BP627627YAG
CSB-MP627627YAG
CSB-EP627627YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Beta-galactosidase (lacZ), partial CSB-YP627628YAG
CSB-EP627628YAG
CSB-BP627628YAG
CSB-MP627628YAG
CSB-EP627628YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Integration host factor subunit alpha (ihfA) CSB-YP627629YAG
CSB-EP627629YAG
CSB-BP627629YAG
CSB-MP627629YAG
CSB-EP627629YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Cysteine desulfurase (sufS) CSB-YP627630YAG
CSB-EP627630YAG
CSB-BP627630YAG
CSB-MP627630YAG
CSB-EP627630YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Pyridoxine/pyridoxamine 5'-phosphate oxidase (pdxH) CSB-YP627631YAG
CSB-EP627631YAG
CSB-BP627631YAG
CSB-MP627631YAG
CSB-EP627631YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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