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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yersinia pestis bv. Antiqua Ribosomal RNA large subunit methyltransferase N (rlmN) CSB-YP625490YAG
CSB-EP625490YAG
CSB-BP625490YAG
CSB-MP625490YAG
CSB-EP625490YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Membrane-bound lytic murein transglycosylase F (mltF) CSB-YP625491YAG
CSB-EP625491YAG
CSB-BP625491YAG
CSB-MP625491YAG
CSB-EP625491YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Ribonuclease 3 (rnc) CSB-YP625492YAG
CSB-EP625492YAG
CSB-BP625492YAG
CSB-MP625492YAG
CSB-EP625492YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Urease accessory protein UreF (ureF) CSB-YP625493YAG
CSB-EP625493YAG
CSB-BP625493YAG
CSB-MP625493YAG
CSB-EP625493YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Probable rRNA maturation factor (YPN_1104) CSB-YP625494YAG
CSB-EP625494YAG
CSB-BP625494YAG
CSB-MP625494YAG
CSB-EP625494YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Lipoyl synthase (lipA) CSB-YP625495YAG
CSB-EP625495YAG
CSB-BP625495YAG
CSB-MP625495YAG
CSB-EP625495YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 50S ribosomal protein L36 (rpmJ) CSB-YP625496YAG
CSB-EP625496YAG
CSB-BP625496YAG
CSB-MP625496YAG
CSB-EP625496YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Trigger factor (tig) CSB-YP625497YAG
CSB-EP625497YAG
CSB-BP625497YAG
CSB-MP625497YAG
CSB-EP625497YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP625498YAG
CSB-EP625498YAG
CSB-BP625498YAG
CSB-MP625498YAG
CSB-EP625498YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Na (+)-translocating NADH-quinone reductase subunit E (nqrE), partial CSB-YP625499YAG1
CSB-EP625499YAG1
CSB-BP625499YAG1
CSB-MP625499YAG1
CSB-EP625499YAG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Ribosome maturation factor RimM (rimM) CSB-YP625500YAG
CSB-EP625500YAG
CSB-BP625500YAG
CSB-MP625500YAG
CSB-EP625500YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF) CSB-YP625501YAG
CSB-EP625501YAG
CSB-BP625501YAG
CSB-MP625501YAG
CSB-EP625501YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Sulfite reductase [NADPH] hemoprotein beta-component (cysI), partial CSB-YP625502YAG
CSB-EP625502YAG
CSB-BP625502YAG
CSB-MP625502YAG
CSB-EP625502YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB) CSB-YP625503YAG
CSB-EP625503YAG
CSB-BP625503YAG
CSB-MP625503YAG
CSB-EP625503YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UPF0231 protein YPN_0674 (YPN_0674) CSB-YP625504YAG
CSB-EP625504YAG
CSB-BP625504YAG
CSB-MP625504YAG
CSB-EP625504YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Lipoprotein NlpI (nlpI) CSB-YP625505YAG
CSB-EP625505YAG
CSB-BP625505YAG
CSB-MP625505YAG
CSB-EP625505YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Glutamate-ammonia-ligase adenylyltransferase (glnE), partial CSB-YP625506YAG
CSB-EP625506YAG
CSB-BP625506YAG
CSB-MP625506YAG
CSB-EP625506YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Serine/threonine transporter sstT (sstT), partial CSB-YP625507YAG
CSB-EP625507YAG
CSB-BP625507YAG
CSB-MP625507YAG
CSB-EP625507YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP625508YAG
CSB-EP625508YAG
CSB-BP625508YAG
CSB-MP625508YAG
CSB-EP625508YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua RNA polymerase-associated protein RapA (rapA), partial CSB-YP625509YAG
CSB-EP625509YAG
CSB-BP625509YAG
CSB-MP625509YAG
CSB-EP625509YAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.bdspa.cn/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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